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Creative Biolabs

Human Retinal Pigment Epithelial Cells (H-RPE)

[CAT#: NCC20-9PZ05] Review(5) Q&As(3)

Cryopreserved ampule of Human Retinal Pigment Epithelial Cells (H-RPE) containing ≥500,000 cells

Species:
Human
Cell Types:
Visual system-related Cells
Cell Subtypes:
Retinal Pigment Epithelial Cells

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Product Overview

Description

Human retinal pigment epithelium (H-RPE) is composed of pigment cells located between the neural retina and choroid. H-RPE is composed of hexagonal cells organized into a single layer, and the single layer is densely packed with pigment particles. H-RPE is of neuroectodermal origin and is therefore considered part of the retina. H-RPE cells play a vital role in visual function and photoreceptor cell viability.

Cell Types

Visual system-related Cells

Cell Subtypes

Retinal Pigment Epithelial Cells

Application Notes

• Guaranteed through 5 population doublings
• Positive immunofluorescence staining for glial fibrillary acid protein (GFAP)

Relevant Diseases

Alzheimer's Disease; Parkinson's Disease

Research Areas

Neurotoxicity; Neurogenesis

Species

Human
Properties

Size

1 x Cryopreserved ampule of H-RPE containing ≥500,000 cells

Form

Cryopreserved

Shipping

Dry Ice

Storage

Liquid nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Quality Control

• Mycoplasma, bacteria, yeast and fungus test negative
• Not all donors and/or cell batches have detected HIV-1, hepatitis B and C
• Certificate of analysis is provided for each cell batch purchased
Donor Information

Donor Health

Normal
Publications

Publications (0)

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Related Products
Scientific Resources
Customer Reviews and Q&As
Customer Reviews Average Customer Ratings Overall
5.0
user
Excellent
Reliable for our research purposes
The cells provided excellent consistency and viability, making them an ideal choice for our retinal research. The cells exhibited strong pigmentation and typical cobblestone morphology, which was critical for our experiments studying the retinal cell layer. The packaging was secure, and the cells arrived promptly in good condition.
user
Excellent
A reliable choice for our retinal cell culture experiments
We used H-RPE cells for our in vitro retinal degeneration model, and they performed exceptionally well. The cells adhered quickly, and their growth rate was consistent with expectations. The cells responded well to experimental treatments, allowing us to generate meaningful data.
user
Excellent
Reliable and consistent
The H-RPE cells we received were of high quality and showed robust cell-cell interactions, which were essential for our study on retinal disease mechanisms. The cells were easy to culture and maintained their phenotype over multiple passages.
user
Excellent
Strong pigmentation and adherence properties
I was impressed by the excellent morphology and confluence of the H-RPE cells. They retained their characteristic pigmentation and formed a uniform monolayer, which was crucial for our drug screening assays targeting retinal conditions. Overall, they met our experimental needs perfectly.
user
Excellent
Easy to handle and responded well to various experimental conditions
We incorporated H-RPE cells into our organoid models, and they integrated seamlessly, displaying expected behavior. The cells were highly viable upon arrival and easy to maintain in culture. The results obtained from our experiments were consistent and reproducible.
Q&As
What is the typical lifespan of these H-RPE cells in culture, and are there any signs I should monitor for cellular senescence?
Under optimal conditions, our H-RPE cells can be maintained for 6-8 passages before significant signs of senescence appear. Indicators such as a change in cell morphology, reduced pigmentation, slower proliferation rates, and increased granularity suggest the onset of cellular aging. We recommend careful monitoring and transitioning to fresh cultures if you observe these signs to ensure consistent experimental results.
Are these H-RPE cells cryopreserved or freshly isolated, and what impact does this have on viability and recovery?
Our H-RPE cells are cryopreserved at an early passage to ensure optimal viability and functionality upon thawing. Cryopreservation allows for convenient storage and ensures that the cells retain their native properties when cultured according to our guidelines. Upon thawing, the cells typically demonstrate high recovery rates and quickly resume their typical cobblestone morphology and pigmentation.
Are the H-RPE cells derived from a healthy donor or a disease model, and can I choose the source based on my research needs?
Our standard H-RPE cells are derived from healthy donors, ensuring the cells retain their normal physiology and morphology. However, we can also provide disease-specific H-RPE cells, such as those associated with age-related macular degeneration (AMD). Please contact us if you need disease model-specific cells for your research, and we can guide you in selecting the best option for your project.
For Research Use Only. Not For Clinical Use.
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