Human Corneal Epithelial Cells, Adult

[CAT#: NCL-2103-P29]

Positive for F4/80

Species:: Human

Cell Types:: Visual system-related Cells

Cell Subtypes:: Corneal Epithelial Cells

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Product Overview

Description

Human corneal epithelial cells (HCEpC) were isolated from the epithelium of the human cornea. They were cryopreserved in the fourth generation, cultivated and reproduced, and reproduced at least 16 times. These cells have been used as a model to study the re-epithelialization of the eye after refractive keratectomy and neutrophil-induced corneal injury. They have also been used to study the activity of matrix metalloproteinases in the corneal epithelium. Human corneal epithelial cells can also be used in research aimed at reconstructing damaged cornea.

Cell Types

Visual system-related Cells

Cell Subtypes

Corneal Epithelial Cells

Species

Human

Properties

Tissue Source

Normal healthy human cornea

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.