Human Retinal Microvascular Endothelial Cells

Species:: Human

Cell Types:: Visual system-related Cells

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Product Overview

Description

HRMECs were isolated from normal human retinal tissue. Cells are shipped frozen at passage 3. To culture the cells, we recommend using Creative Biolabs' endogenous growth medium. These cells have a minimum average population doubling level > 20 when cultured following our protocol.
HRMECs are negative for HIV-1, HBV, HCV and Mycoplasma.

Cell Types

Visual system-related Cells

Species

Human

Properties

Size

500,000+ Cells Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Frozen (Dry Ice/Liquid Nitrogen)

Storage

Keep frozen in liquid nitrogen until plating

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Donor Information

Donor Health

Normal

Publications

Publications (0)

gift-card

Related Products

Neural Cell Lines

Rat Neonatal Retinal Cells

Human Corneal Epithelial Cells, Adult

Human Retinal Microvascular Endothelial Cells

Porcine Trabecular Meshwork Cells

Human Trabecular Meshwork Cells, Adult

Human Retinal Pigment Epithelial Cells (H-RPE)

Human Retinal Pigment Epithelial Cells

Rabbit Corneal Epithelial Cells

Human Retinal Microvascular Pericytes (HRMPVCs)

Canine Corneal Keratocytes

Scientific Resources

[Brochure]

Neuros Celll Product Panel

View

Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

These cells have been a cornerstone in my recent research

I was particularly impressed with their viability and growth rate. They responded well to my experimental treatments and provided reliable data. The customer support was also very helpful in answering my initial questions.

Excellent

The cells are robust and consistent, showing excellent morphology and proliferation

They express all the expected markers, which has been crucial for my immunofluorescence studies. The technical support provided was top-notch, helping me optimize my culture conditions.

Excellent

The cells from this supplier have exceeded my expectations

The cells were shipped quickly and were easy to thaw and culture. They maintained high viability and purity, which was essential for my in vitro assays. I particularly appreciated the detailed protocols provided, which made the initial setup smooth and efficient.

Excellent

I highly recommend these cells

I've had a great experience using these cells in my retinal disease models. They exhibit reliable growth patterns and maintain their endothelial characteristics throughout multiple passages. The ability to consistently produce tight junctions in my assays has been invaluable.

Excellent

Performed exceptionally well

I have used them in various permeability assays and found them to be very responsive and stable. The cells arrived in excellent condition, and the provided documentation was thorough, making it easy to integrate them into my experimental workflows.

Q&As

What quality control measures are in place for HRMECs provided by your company?

Our HRMECs undergo rigorous quality control measures to ensure their reliability and consistency. This includes checking for cell viability, morphology, and marker expression. We also perform tests for endotoxin levels and microbial contamination. Each batch of HRMECs is thoroughly characterized to confirm their endothelial phenotype and functionality before being released for research use.

How long can HRMECs be maintained in culture?

HRMECs can typically be maintained in culture for a certain number of passages, usually up to 6-8 passages, before their growth rate and functionality may begin to decline. Regular monitoring of cell morphology and functionality is necessary to ensure that they remain suitable for experiments. Proper culture conditions and handling can help maximize their lifespan and research utility.

Can HRMECs be used to study blood-retina barrier integrity?

Yes, HRMECs are an excellent model for studying blood-retina barrier integrity. They can be used to investigate the impact of various factors on the permeability of the blood-retina barrier, such as inflammatory cytokines or oxidative stress. Researchers use these cells to assess barrier function, tight junction protein expression, and changes in permeability, which are crucial for understanding retinal vascular diseases.