Canine Corneal Keratocytes

[CAT#: NCL-2103-P33]

Species:: Dog

Cell Types:: Visual system-related Cells

Cell Subtypes:: Corneal Keratocytes

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Product Overview

Description

Canine corneal keratocytes (CnCK) are primary cells derived from canine eyes. Corneal keratocytes are neural-derived mesenchymal cells distributed in the corneal stroma. The matrix is composed of type I and type V collagen fibers arranged in Flake-shaped, predominantly. When the cornea is injured, it stimulates corneal cells to undergo apoptosis or transform into a repair phenotype to promote corneal regeneration.

When cultured under recommended conditions, canine corneal corneal cells (CnCK) cultured in corneal corneal cell growth medium can multiply to 10 population doublings. CnCK can be used as an important in vitro cell model for the study of corneal cell differentiation, corneal injury and related diseases.

Cell Types

Visual system-related Cells

Cell Subtypes

Corneal Keratocytes

Species

Dog

Properties

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.