NeuroMab™ Anti-Notch1 BBB Shuttle Antibody(NRZP-1022-ZP3211)

Figure 1 is a histogram summarizing the results of experiments to determine the specificity of antibodies binding to human Notch 1 on the surface of CHO-Flpln-Notch 1 cells. Antibodies 2G10, 2E6 (also referred to herein as antibody 2E06), 2A11 and 2D11 are shown from left to right.

Figure 2 is a bar graph summarizing the results from a Notch3-specific reporter assay showing that antibody 2E6 does not inhibit Notch3-dependent reporter expression in the presence of Jag1.

Figure 3 is a graph showing DBZ (dibenzozapine; a γ-secretase inhibitor once daily at a dose of 10 μmol/kg) and antibody 2E6 (three times weekly at a dose of 40, 100 or 150 mg/kg (abbreviated as “ mpk"))) on mouse thymocyte populations.

Figure 4 is a histogram summarizing data from experiments evaluating the effect of antibody 2E6 on bFGF-induced functional angiogenesis in vivo, where hemoglobin levels were used as a surrogate indicator of functional angiogenesis.

Figure 5 is a histogram summarizing data from experiments evaluating the effect of antibody 2E6 on functional angiogenesis induced by human cancer cell lines in vivo

Figure 6 is a graph summarizing the results of a Notch-1-specific reporter assay showing antibodies mu2E6 (■), Hu2E6-62 (A), A2-NRR1 (T), and a mouse IgG control (·).

Figure 7 is a graph showing the change in mouse body weight over time for mice treated with antibodies Hu2E6-62 (■), A2-NRR1 (A) and human IgG (■) control.

Figure 8 is a photograph showing alcian blue staining of sections of small intestine after treatment with antibodies Hu2E6-62, A2-NRR1 and human IgG control.

Figure 9 is a histogram summarizing data from experiments evaluating the effect of antibody Hu2E6-62 on bFGF-induced functional angiogenesis in vivo, where hemoglobin levels were used as a surrogate for functional angiogenesis.

Figure 10 is a histogram summarizing data from experiments evaluating the effect of antibody Hu2E6-62 on functional angiogenesis induced by the human lung cancer cell line Calu-6 in vivo.