NeuroMab™ Anti-TDP43 Antibody(NRP-0422-P713)

Figure 1 shows the density of pTDP-43 immunoreactive objects measured for vehicle (n = 30, gray bars) and ACI-7069-633B12-AM (IgG2a variant)-treated mice (n = 25, dotted gray bars) for the two brain regions.

Immunofluorescent staining of human TDP-43 and TDP-43 oligomers in brain sections of wild-type and 6- and 12-month-old TDP-43 Tg+/+ transgenic mice. Cells with anti-TDP oligomer staining, TDP-43 staining, and DAPI staining are shown (scale bar, 100 μm). Closed regions are presented at higher magnification in the last column (scale bar, 25 μm).

Figure 2 TEV cleavage-induced aggregation of TDP-43 in the presence of ACI-7069-633B12-AM (IgG2a variant) or an isotype control measured after 30 hours by turbidity at 600 nm.

The endpoint after 30 h was normalized to the isotype control (gray bars) and the percent aggregated TDP-43 was calculated for ACI-7069-633B12-AM (dashed gray bars). Mean ± SD of three independent experiments are shown and statistical differences between isotype control and ACI-7069-633B12-AM (IgG2a variant) were analyzed by Welch's t-test (*** p < 0.001).

Figure 3. Ibal-positive immunoreactive areas measured for vehicle (n = 16, gray bars) and ACI-7069-633B12-AM (IgG2a variant) treated mice (n = 16, gray dotted bars) showing cerebral cortex. Error bars represent standard error of the mean (SEM).

The endpoint after 30 h was normalized to the isotype control (gray bars) and the percent aggregated TDP-43 was calculated for ACI-7069-633B12-AM (dashed gray bars). Mean ± SD of three independent experiments are shown and statistical differences between isotype control and ACI-7069-633B12-AM (IgG2a variant) were analyzed by Welch's t-test (*** p < 0.001).

Figure 4 TDP-43 levels in CSF of various FTLD-TDP patients and healthy controls using the AlphaLISA assay for ACI-7069-633B12-AM (IgG2a variant) and ACI-7071-809F12-Abl (IgG2a variant) Quantitative.

The endpoint after 30 h was normalized to the isotype control (gray bars) and the percent aggregated TDP-43 was calculated for ACI-7069-633B12-AM (dashed gray bars). Mean ± SD of three independent experiments are shown and statistical differences between isotype control and ACI-7069-633B12-AM (IgG2a variant) were analyzed by Welch's t-test (*** p < 0.001).

Figure 5 Immunodepletion of TDP-43 and pTDP-43 by antibodies ACI-7069-633B12-AM (IgG2a variant) (1), ACI-7069-642D12-AM (IgG2a variant).

The endpoint after 30 h was normalized to the isotype control (gray bars) and the percent aggregated TDP-43 was calculated for ACI-7069-633B12-AM (dashed gray bars). Mean ± SD of three independent experiments are shown and statistical differences between isotype control and ACI-7069-633B12-AM (IgG2a variant) were analyzed by Welch's t-test (*** p < 0.001).