NeuroMab™ Mouse Anti-SOX2 Monoclonal Antibody (CBP2177)

Product Overview

Description

SOX2 is the most studied member of SRY-related box transcription factor family. It binds to target genes through its highly conserved HMG box domain. Inactivation of the SOX2 gene causes lethality during embryonic development. SOX2 knockdown in embryonic stem cells results in their differentiation. Co-expression of SOX with OCT4, MYC, and KLF4 is sufficient to reprogram somatic cells to induced pluripotent stem cells (iPSCs), which exert similar characteristics as natural pluripotent stem cells. These findings indicate that SOX2 is crucial for the self-renewal and pluripotency of embryonic stem cells. In addition, over-expression of SOX2 has been found in various types of malignant cancer. Knockdown of SOX2 results in cell cycle arrest by downregulating cyclin D1 and inhibition of tumor cell proliferation, suggesting that SOX2 is involved in activating genes associated with tumor progression.

Immunogen

Full length human SOX recombinant protein

Species Reactivity

Human; Mouse

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2177

Applications

FC; ICC

Application Notes

ICC (1.0-5.0 μg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1617

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Handling Advice

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

SOX2

Official Name

SOX2

Full Name

SRY-Box 2

Alternative Names

SRY-related HMG-box gene 2; SRY (sex determining region Y)-box 2; MCOPS3; ANOP3

Gene ID

6657(Human); 20674(Mouse)

Uniprot ID

P48431(Human); P48432(Mouse)

References

1. Rizzino A. 2009. Wiley Interdiscip. Rev. Syst. Biol. Med. 1:228.
2. Stolzenburg S, et al. 2012. Nucleic Acids Res. 40:6725.
3. Lai YS, et al. 2012. Proc. Natl. Acad. Sci. USA. 109:3772.
4. Jeong CH, et al. 2010. Stem Cells 28:2141.
5. Xiang R, et al. 2011. Br. J. Cancer 104:1410.
6. Card DA, et al. 2008. Mol. Cell Biol. 28:6426.

Publications