VPAC1 Receptor Assay Cell Line
[CAT#: NCL2110P261]
- Species:
- Human
- Cell Types:
- Other Cells
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Cell line information: VIPR1 cAMP Maxensor cell line
Official Name: Vasoactive intestinal peptide receptor 1
DNA Accession Number: BC064424
Host Cell: U2OS
Resistance: G418 + Puromycin
Quantity:> 3 x 10^6 cells / vial
Storage: Liquid Nitrogen
Official Name: Vasoactive intestinal peptide receptor 1
DNA Accession Number: BC064424
Host Cell: U2OS
Resistance: G418 + Puromycin
Quantity:> 3 x 10^6 cells / vial
Storage: Liquid Nitrogen
Features
The red cAMP Maxensor-VIPR1 contains U2OS cells stably expressing the red cAMP Maxensor biosensor and vasoactive intestinal peptide receptor 1 (unlabeled). Creative Biolabs' red cAMP Maxensor-VIPR1 cell line is designed to test compounds or analyze their ability to modulate vasoactive intestinal peptide receptor 1. When the agonist binds to VIPR1, the G protein is activated, which in turn triggers the camp. This cell line has been verified by measuring the increase of cAMP in the cytosol and analyzing the distribution of the red cAMP Maxensor biosensor in the cell. This cell line allows image analysis of compound-induced stimuli. In high-content analysis (HCA) and high-throughput analysis (HTA), the use of human PACAP-38 as an agonist validates this highly reproducible assay.
Cell Types
Other Cells
Relevant Diseases
Amyotrophic Lateral Sclerosis; CNS
Assay
VPAC1 Receptor Assay
Assay Description
The VPAC1 receptor detection (VIPR1) from Creative Biolabs uses Maxensor Biosensors technology to measure VIPR1 activation. VPAC1 receptor signal is stimulated by adenylate cyclase through the trimeric Gs protein, increased cAMP and downstream L-type calcium channel interactions.
In this assay, we use the cAMP-Maxensor biosensor to measure receptor activation/inhibition through cAMP accumulation. The high reproducibility of this assay allows monitoring of the VPAC1 receptor activation process in living cells.
In this assay, we use the cAMP-Maxensor biosensor to measure receptor activation/inhibition through cAMP accumulation. The high reproducibility of this assay allows monitoring of the VPAC1 receptor activation process in living cells.
Assay Characteristics
Readout: cAMP Flux (Gs activation)
Agonist: PACAP-38
EC50 Agonist: 2.47 x 106-9 M for cAMP Flux
Type of Assay: Cell-Based/Functional
Detection Method: Fluorimetry
Agonist: PACAP-38
EC50 Agonist: 2.47 x 106-9 M for cAMP Flux
Type of Assay: Cell-Based/Functional
Detection Method: Fluorimetry
Species
Human
Properties
Form
Frozen
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
No bacteria, yeast, fungi, mycoplasma, virus
Target Details
Target
VIPR1
Full Name
Vpac1 Receptor
Alternative Names
VIPR1; HVR1; II; PACAP-R-2; PACAP-R2; RDC1; V1RG; VAPC1; VIP-R-1; VIPR; VIRG; VPAC1; VPAC1R; VPCAP1R; vasoactive intestinal peptide receptor 1
Publications
Publications (0)
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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