Human CHO-K1/DP2/Gα15 Stable Cell Line

[CAT#: NCL20120115CR]

Prostanoid Receptor DP2 Stable Cell Line

Species:: Human

Applications:: GPCR Screening

Cell Types:: Other Cells

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Product Overview

Description

It is derived from arachidonic acid and binds with high affinity to the G-protein coupled receptors prostanoid DP(1) and DP(2). Interaction with DP(2) results in cell chemotaxis, eosinophil degranulation, eosinophil shape change, adhesion molecule upregulation and Th2 cytokine production. In allergic rhinitis and allergic asthma PGD(2) is released from mast cells in response to allergen challenge and may trigger symptoms such as sneezing, rhinorrhea, pruritus, mucus hypersecretion and pulmonary inflammation.

Cell Types

Other Cells

Applications

GPCR Screening

Research Areas

GPCR; Neural Signal Transduction

Species

Human

Properties

Size

>1x10^6 Cells

Form

Frozen cells

Culture Medium

Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 400 μg/ml G418

Freeze Medium

45% culture medium, 45% FBS, 10% DMSO

Receptor

DP2

Family

Prostanoid receptor

Strain

DP2/Gα15 Cell Line

Cell Purity

>95%

Shipping

Dry ice

Storage

Liquid nitrogen immediately upon delivery

Handling Advice

Avoid inhalation. Avoid contact with eyes, skin, and clothing. Avoid prolonged or
repeated exposure.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents.

Target Details

Target

DP2

Official Name

DP2

Alternative Names

DP2 receptor; CRTH2; GPR44; CD294; DP2; G protein-coupled receptor 44; chemoattractant receptor homologous molecule expressed on T helper type 2 cells; Gpr44_predicted; LOC309212; G protein-coupled receptor 44 (predicted); prostaglandin D2 receptor 2; PGD2 receptor

Gene ID

7029(Human); 211586(Mouse)

Uniprot ID

Q14188(Human); Q64163(Mouse)

References

1. Montagna, E., Crux, S., Luckner, M., Herber, J., Colombo, A. V., Marinković, P.,.. & Sgobio, C. (2019). In vivo Ca2+ imaging of astrocytic microdomains reveals a critical role of the amyloid precursor protein for mitochondria. Glia, 67(5), 985-998. 2. Xu, Y., Wu, X., Wang, S., Yang, C., Li, Y., & Cao, Y. (2019). Hydroxyapatite Nanoparticle-Crosslinked Peptide Hydrogels for Three-Dimensional Culture and Differentiation of MC3T3-E1 Osteoblasts. Journal of biomedical nanotechnology, 15(12), 2351-2362. 3. Lan, J., Rollins, J. A., Zang, X., Wu, D., Zou, L., Wang, Z.,.. & Chen, D. (2019). Translational regulation of non-autonomous mitochondrial stress response promotes longevity. Cell reports, 28(4), 1050-1062.

Publications