NeuroMab™ Anti-CXCR4 BBB Shuttle Antibody(NRZP-1022-ZP3804)

Figure 1 is a series of FACS analyzes demonstrating that anti-CXCR4 scFvs 33 and 48 specifically bind CXCR4. However, these scFvs do not bind the closely related chemokine receptor CCR5.

Figure 2 is a series of FACS analysis graphs showing that scFvs 48 binds to Cf2ThCD4+CXCR4+ cells but not to parental Cf2Th or Cf2ThCD4+CCR5+ cells.

Figure 3 is a series of FACS scans showing that scFvs 33 and 48 do not cross-react with other chemokine receptors such as CXCR6, CXCR1, GPR15 and CXCR2.

Figure 4 is a FACS scan analysis showing that scFv clones 33 and 48 compete with neutralizing anti-CXCR4 monoclonal antibody 12G5 for binding to CXCR4.

Figure 5 shows the results of FACS analysis of the binding of 33 scFv-Fc and 48 scFv-Fc to 293T cells expressing human, mouse and rhesus monkey CXCR4.

Figure 6 is a series of graphs demonstrating inhibition of HIV-1 reporter virus entry into Cf2ThCD4CXCR4 cells.

Figure 7 is a series of graphs showing inhibition of HIV-1 reporter virus entry by scFvs 33 and 48.

Figure 8 is a graph showing the effect of scFvs 33 and 48 on SDF-1 mediated chemotaxis of Jurkat T cells.