NeuroMab™ Anti-CXCR4 BBB Shuttle Antibody(NRZP-1022-ZP3808)

Figure 6 is a series of graphs showing the titration of anti-CXCR4 scFv-Fc fusion protein with Jurkat and cF2ThCXCR4C9 cell lines.

Figure 7 is a series of graphs showing the titration of anti-CXCR4 scFv-Fc fusion protein with Jurkat and cF2ThCXCR4C9 cell lines.

Figure 1 shows the titration of anti-CXCR4 scFv-Fc fusion protein with Jurkat cell line.

Figure 2 is a series of graphs demonstrating the ability of scFv fusion proteins to block entry of a single round of pseudotyped HIV-1 into target cells.

Luciferase activity (cpm) was measured after 48 hours. Inhibitory capacity was calculated as the average of triplicate wells: (average cpm of experimental wells/average cpm of wells not treated with antibody before virus addition) × 100%. The X4 tropical HIV-1 pseudovirus Hxbc-2 was tested in this experiment.

Figure 3 is a histogram showing the downregulation of cell surface expressed CXCR4 protein.

Figure 4 is a series of FACS analysis graphs showing that X33, X48, X18, X19 and X20 specifically bind to CXCR4 cells.

Figure 5 is a graph of FACS analysis demonstrating that X18, X19 and X20 (except X33 and X48) compete with 12G5 for binding to CXCR4.

Figure 6 is a series of graphs showing the titration of anti-CXCR4 scFv-Fc fusion protein with Jurkat and cF2ThCXCR4C9 cell lines.

Figure 7 is a series of graphs showing the titration of anti-CXCR4 scFv-Fc fusion protein with Jurkat and cF2ThCXCR4C9 cell lines.