NeuroMab™ Anti-SPP1 Antibody(NRP-0422-P692)

Product Overview

Description

The antibody recognizes a transglutamination site of osteopontin that inhibits the accumulation of neutrophils, and inhibits the polymerization of osteopontin.

Species Reactivity

Human; Mouse

Clonality

Monoclonal

Host Species

Chicken

Applications

WB; ELISA; Block; Inhib; In Vitro

Relevant Diseases

Alzheimer's Disease; Neuroinflammation

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

SPP1

Official Name

SPP1

Full Name

Secreted phosphoprotein 1

Alternative Names

BNSP;
Bone sialoprotein 1;
Bone sialoprotein I;
BSP I;
BSPI;
Early T lymphocyte activation 1;
ETA 1;
ETA1;
MGC110940;
Nephropontin;
OPN;
Osteopontin;

Gene ID

6696(Human)

Uniprot ID

P10451(Human)

Product Pictures

ELISA

Figure 1 shows the results of an ELISA survey of the OPN locus recognized by HUPB1 chimeric IgG.

EC50 values were calculated from a 4 parameter fit equation using Prism Graphpad using the data from Figure 10. A2=2.6 ng/ml, A6=3.2 ng/ml; E5=4.0 ng/ml, G7=93.2 ng/ml.

ELISA

Figure 2 shows the results of examining the binding properties of HUPB1 chimeric IgG to monomeric OPN and polymeric OPN by ELISA.

EC50 values were calculated from a 4 parameter fit equation using Prism Graphpad using the data from Figure 10. A2=2.6 ng/ml, A6=3.2 ng/ml; E5=4.0 ng/ml, G7=93.2 ng/ml.

Figure 3 shows the results of examining the inhibitory effect of HUPB1B chimeric IgG on OPN polymerization by Western blotting.

EC50 values were calculated from a 4 parameter fit equation using Prism Graphpad using the data from Figure 10. A2=2.6 ng/ml, A6=3.2 ng/ml; E5=4.0 ng/ml, G7=93.2 ng/ml.

Inhib

Figure 4 shows the results of studying the inhibitory activity of HUPB1 chimeric IgG on neutrophil aggregation.

EC50 values were calculated from a 4 parameter fit equation using Prism Graphpad using the data from Figure 10. A2=2.6 ng/ml, A6=3.2 ng/ml; E5=4.0 ng/ml, G7=93.2 ng/ml.

Publications