NeuroMab™ Mouse Anti-Human ITGAM Monoclonal Antibody (CBP1542)

Description

Human CD147 cell surface antigen, also known as Basigin, EMMPRIN and collagenase stimulating factor. CD147 is a single-pass type 1 transmembrane glycoprotein of 385 amino acids, with 3 potential N-glycosylation sites, a single N-terminal (distal) Ig-like C2-type domain and a more proximal Ig-like domain V-domain extracellular region. Produce mouse anti-human CD147 antibody, clone MEM-M6/1, and screen against the recombinant extracellular domain of huCD147 expressed in CHO cells and many other MEM-M6 clones. Epitope mapping shows that clone MEM-M6/1 binds to epitopes in the distal Ig-like C-2 type domain. This was confirmed by flow cytometry studies using construct-coated beads containing only distal Ig-like or proximal Ig-like domains.

Immunogen

The extracellular region of human CD147.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP1542

Applications

FC; IP; WB

Application Notes

FCM (1/50-1/100)

Research Areas

Growth and Development

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1344

Form

Liquid

Formulation

PBS
0.09% Sodium Azide

Concentration

1 mg/ml

Purification

Purified IgG prepared by Protein A affinity chromatography.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C only. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

CD11b

Official Name

ITGAM

Full Name

Integrin alpha M

Alternative Names

ITGAM; CD11B; CR3A; MAC-1; MAC1A; MO1A; SLEB6; integrin subunit alpha M

Gene ID

3684 (Human); 16409 (Mouse); 25021 (Rat)

Uniprot ID

P11215 (Human); P05555 (Mouse)

1. Koch, C. et al. (1999) T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density. 2. Yang, Y. et al. (2008) Cyclophilin A up-regulates MMP-9 expression and adhesion of monocytes/macrophages via CD147 signalling pathway in rheumatoid arthritis. 3. Schneiderhan, W. et al. (2007) Pancreatic stellate cells are an important source of MMP-2 in human pancreatic cancer and accelerate tumor progression in a murine xenograft model and CAM assay. 4. Chen, Y. et al. (2009) Upregulation of HAb18G/CD147 in activated human umbilical vein endothelial cells enhances the angiogenesis. 5. Schneiderhan, W. et al. (2009) CD147 silencing inhibits lactate transport and reduces malignant potential of pancreatic cancer cells in in vivo and in vitro models 6. Wright, G.J. et al. (2014) Immunogenic compositions and expression systems. 7. Huang, Z. et al. (2013) Overexpression of CD147 contributes to the chemoresistance of head and neck squamous cell carcinoma cells. 8. Hu, J. et al. (2010) Involvement of HAb18G/CD147 in T cell activation and immunological synapse formation. 9. Bernard, S.C. et al. (2014) Pathogenic Neisseria meningitidis utilizes CD147 for vascular colonization. 10. Nassif, X. et al. (2015) Cd147 as receptor for pilus-mediated adhesion of meningococci to vascular endothelia. 11. Trakarnsanga, K. et al. (2017) An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.