NeuroMab™ Mouse Anti-ITGAM Monoclonal Antibody (CBP1529)

Description

It recognizes human integrin beta1, also known as CD29, fibronectin receptor subunit beta, glycoprotein IIa or VLA-4 subunit beta. CD29 is a single-pass type 1 transmembrane glycoprotein containing 798 amino acids and a single VWFA domain of approximately 130 kDa. The specificity for CD29 has been confirmed with the integrin β1 transfectant and the β1 reactivity with the anti-β1 antibody pre-clearing each other.

Immunogen

Human beta1 integrin.

Species Reactivity

Human; Porcine; Cynomolgus monkey; Rhesus Monkey

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2a

Clone Number

CBP1529

Applications

ELISA; FC; IHC-Fr; IP; WB

Application Notes

ELISA (0.1ug/ml-1ug/ml); FCM (1ug/10⁶cells); IHC-Fr (5ug/ml); IP (1-5ug)

Research Areas

Growth and Development

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1336

Form

Liquid

Formulation

PBS
0.09% Sodium Azide

Concentration

0.5 mg/ml

Purification

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

CD11b

Official Name

ITGAM

Full Name

Integrin alpha M

Alternative Names

ITGAM; CD11B; CR3A; MAC-1; MAC1A; MO1A; SLEB6; integrin subunit alpha M

Gene ID

3684 (Human); 16409 (Mouse); 25021 (Rat)

Uniprot ID

P11215 (Human); P05555 (Mouse)

1. Stupack, D.G. et al. (1994) Control of lymphocyte integrin function: evidence for multiple contributing factors. 2. Wilkins, J.A. et al. (1996) Control of beta1 integrin function. Localization of stimulatory epitopes. 3. Yoshino N et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. 4. Batista da Silva, A.P. et al. (2004) The major outer sheath protein of Treponema denticola inhibits the binding step of collagen phagocytosis in fibroblasts. 5. Levite, M. et al. (2000) Extracellular K(+) and opening of voltage-gated potassium channels activate T cell integrin function: physical and functional association between Kv1.3 channels and beta1 integrins. 6. Bass, R. et al. (2005) Regulation of urokinase receptor proteolytic function by the tetraspanin CD82. 7. Clark, K. et al. (2000) Production of recombinant soluble human integrin alpha4beta1. 8. Papageorgiou, I. et al. (2014) Interaction of micron and nano-sized particles with cells of the dura mater. 9. Takaki, T. (2006) Interferon-gamma inhibits collagen phagocytosis in human fibroblasts by inducing subcortical actin assembly and reducing ability of beta1 integrin to bind to collagen. 10. Eirin A et al. (2017) Integrated transcriptomic and proteomic analysis of the molecular cargo of extracellular vesicles derived from porcine adipose tissue-derived mesenchymal stem cells. 11. Meng, Y. et al. (2018) The metabolic syndrome alters the miRNA signature of porcine adipose tissue-derived mesenchymal stem cells. 12. Dawson, H.D. and Lunney, J.K. (2018) Porcine cluster of differentiation (CD) markers 2018 update.