Rat Midbrain Neurons

Description

Rat midbrain neurons (RMbN) are obtained from the midbrain of normal embryonic rats (the 15th day of pregnancy) by a standardized method. When cultured under the recommended conditions, RMbN forms a tree shape and complex formation within one week. The neurite network. RMBN is positive for βIII-tubulin.
The midbrain is a small part of the brain, but plays an important role in the visual and auditory systems, motor control, alertness, sleep/wake and temperature regulation. It contains the substantia nigra, which is a dark area with a large number of dopaminergic neurons, which is closely related to motivation and habits. Midbrain atrophy and diseases seen in diseases such as Parkinson's disease, familial amyotrophic lateral sclerosis, and progressive supranuclear palsy can affect balance, gait, and muscle control. Studying midbrain neurons under those disease conditions will enable people to better understand disease mechanisms, physiopathology, and progress in drug development and treatment. The culture of midbrain neurons can be used in a variety of experiments, including toxicity tests, immunostaining, live cell imaging, co-culture, electrophysiology, etc.

Cell Types

Neurons

Species

Rat

Tissue Source

Normal healthy rat midbrain

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

No bacteria, yeast, fungi, mycoplasma, virus

Donor Health

Normal