Primary Human Neurons

Species:: Human

Cell Types:: Neurons

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Product Overview

Description

Human primary neurons are activated by collagenase digesting chopped cerebral cortex tissue. Human neurons are separated from the mixed cell population and placed in frozen vials at the passage 3. HNCs growth medium (containing 10% serum) and growth supplements, cat# NCC-2103-P63 is recommended for cell culture.

Cell Types

Neurons

Species

Human

Properties

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Frozen (Dry Ice/Liquid Nitrogen)

Storage

Keep frozen in liquid nitrogen until plating

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Donor Information

Donor Health

Normal

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Stability and ease of maintenance

The neurons provided were of excellent quality and consistency. They cultured well and exhibited typical neuronal morphology, which was crucial for our studies on synaptic function.

Excellent

Good neuronal differentiation and functional properties

We were very pleased with the viability and purity of the primary human neurons. They performed well in our electrophysiology assays, showing robust responses that aligned with our experimental needs.

Excellent

Highly viable and integrated well into our existing cellular models

The neurons arrived well-preserved and were easy to handle. They integrated seamlessly into our neuronal networks for synaptic plasticity studies. A reliable choice for those needing human neuronal models.

Excellent

Easy to handle and showed good consistency

They showed clear, consistent responses to various neurotoxic compounds, proving to be a valuable tool in our research.

Excellent

They exhibited appropriate connectivity and activity patterns

They were crucial for our work on neurodegenerative diseases, offering reproducible results and clear markers of disease progression.

Q&As

How many passages before these cell become senescent?

HN cannot be subcultured or passaged, as the cells do not proliferate.

How long can human neurons be cultured before they need to be replaced?

Primary human neurons can typically be cultured for several weeks, depending on the specific experimental needs and conditions. It's crucial to monitor neuronal health regularly through morphological assessments and viability assays. If you observe significant changes in cell morphology or decreased viability, it's advisable to replace the cultures or refresh the medium to maintain experimental accuracy.

Are there any specific protocols for thawing and plating primary human neurons?

When thawing neurons, do so quickly in a 37°C water bath and then transfer them to pre-warmed culture medium. Gently centrifuge and resuspend the cells in an appropriate volume of culture medium. Plate the neurons at the recommended density on coated culture dishes to support cell adhesion and growth. Follow the detailed protocol provided with the product for best results.

Are there any specific considerations for using primary human neurons in co-culture systems?

When using neurons in co-culture systems, ensure that the co-cultured cell types are compatible and that the conditions support the survival and function of all cell types involved. It’s important to optimize the ratios of neuronal to non-neuronal cells and to provide appropriate growth factors and extracellular matrix components. Regularly monitor the interactions between cell types to ensure accurate experimental outcomes.