- Host Species:
- Mouse
- Species Reactivity:
- Human; Mouse
- Applications:
- FC; In Vitro; In Vivo; Agonist; WB
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inquiryExpression Host
Species Reactivity
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Low Endotoxin < 1 EU/mg
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Research Use Only
Figure 1 shows Syk phosphorylation determined by Western blot analysis in mouse bone marrow-derived macrophages following incubation with TREM2 antibodies 2F6, 11H5, 2H8, 1H7, 3A7, 3B10, 10A9, 7F8, and 7E5.
Figure 3 shows the induction of a mouse TREM2-dependent luciferase reporter gene in a cell-based assay.
Cells were either untreated (NT) or treated with soluble full-length anti-TREM2 antibodies 1H7, 2F6, 2H8, 3A7, 3B10, 7E5, 7F8, 8F8, and 11H5. Antibody mlgG1 is an isotype negative control. Cells treated with PMA/ionomycin represent a positive control. Results are represented as folded backgrounds (indicated by dashed lines).
Figure 4 shows the percentage of neutrophils in the peritoneal cavity after injection of LPS, control (CTR) or TREM2 antibody 7E5 alone or CTR or TREM2 antibody 7E5 with LPS.
Figure 5 shows the concentration of CCL4 (pg/ml) in the peritoneal cavity after injection of control (CTR) or TREM2 antibodies 7E5 and 8F8 in combination with LPS.
Figure 6 shows the mean concentration (ug/ml) of 7E5 antibody found in serum on days 2, 4, 8 and 15 after intraperitoneal injection of the indicated doses of antibody from three mice.
Measurement of soluble 7E5 antibody was performed by standard ELISA. Data were analyzed with Prism6 software and exponential monophasic decay curves were fitted to calculate half-lives. The half-life of the antibody in mouse serum is approximately 9.5 days.
Figure 7 shows the BMS subscores. The results showed that antibody 7E5 caused a transient improvement in motor function after spinal cord contusion, as measured by the BMS scoring system. *p<0.05, 2-way repeated ANOVA with Tukey's post hoc test.
Figure 8 shows the percent survival (%) of human monocyte-derived dendritic cells incubated with soluble TREM2 antibodies 9F5 or 10A9.
In contrast to antibody 10A9, the survival of dendritic cells was not significantly reduced after incubation with antibody 9F5. "mlgG1" refers to the mouse isotype control antibody, and "medium" refers to the media-only control.
Figure 9 shows that treatment of chronically challenged mice with dextran sodium sulfate (DSS) and anti-TREM2 antibody 7E5 significantly reduces the symptoms of chronic colitis.
Shows weight loss in chronic DSS-challenged mice treated with antibody 7E5
Publications (0)
ExcellentA versatile toolThis anti-TREM2 antibody worked exceptionally well in our Western blot assays. The signal was strong, and the specificity was impressive, with minimal background noise. It also performed well in flow cytometry, providing clear differentiation between TREM2-positive and negative cells.
ExcellentA reliable product for TREM2 researchWe used this antibody for in vitro experiments and were pleased with the results. The antibody was effective in recognizing TREM2 in both cell lysates and live cells. It also demonstrated good agonistic properties, which was critical for our study.
ExcellentA solid choice for any TREM2-related studyI tested this antibody in a flow cytometry experiment, and the results were satisfactory. The staining was crisp, and we didn't observe any nonspecific binding.
ExcellentA high-quality productOur lab used the anti-TREM2 antibody in Western blotting and found it to be of high quality. The antibody provided clear and consistent bands, even at low concentrations. It was also effective in flow cytometry, offering precise detection of TREM2.
ExcellentHighly recommendedThe staining was specific and reproducible across multiple samples. We also used it in some in vitro functional assays and saw significant results. This antibody is definitely worth the investment for TREM2-related studies.
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