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Creative Biolabs

NeuroMab™ Anti-TREM2 Antibody(NRP-0422-P1400)

[CAT#: NRP-0422-P1400] Review(5) Q&As(3)

Functional antibody against Human TREM2

Host Species:
Mouse
Species Reactivity:
Human; Mouse
Applications:
FC; In Vitro; In Vivo; Agonist; WB

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Product Overview

Expression Host

Hybridoma

Species Reactivity

Human; Mouse

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Applications

FC; In Vitro; In Vivo; Agonist; WB

Relevant Diseases

Alzheimer's Disease
Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only
Target

Target

TREM2

Official Name

TREM2

Full Name

Triggering receptor expressed on myeloid cells 2

Alternative Names

TREM2; Triggering receptor expressed on myeloid cells 2

Gene ID

54209(Human)

Uniprot ID

Q9NZC2(Human)
Publications

Publications (0)

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Customer Reviews and Q&As
Customer Reviews Average Customer Ratings Overall
5.0
user
Excellent
A versatile tool
This anti-TREM2 antibody worked exceptionally well in our Western blot assays. The signal was strong, and the specificity was impressive, with minimal background noise. It also performed well in flow cytometry, providing clear differentiation between TREM2-positive and negative cells.
user
Excellent
A reliable product for TREM2 research
We used this antibody for in vitro experiments and were pleased with the results. The antibody was effective in recognizing TREM2 in both cell lysates and live cells. It also demonstrated good agonistic properties, which was critical for our study.
user
Excellent
A solid choice for any TREM2-related study
I tested this antibody in a flow cytometry experiment, and the results were satisfactory. The staining was crisp, and we didn't observe any nonspecific binding.
user
Excellent
A high-quality product
Our lab used the anti-TREM2 antibody in Western blotting and found it to be of high quality. The antibody provided clear and consistent bands, even at low concentrations. It was also effective in flow cytometry, offering precise detection of TREM2.
user
Excellent
Highly recommended
The staining was specific and reproducible across multiple samples. We also used it in some in vitro functional assays and saw significant results. This antibody is definitely worth the investment for TREM2-related studies.
Q&As
Can this antibody be used as an agonist in in vitro experiments?
Yes, this anti-TREM2 antibody has been shown to function as an agonist in in vitro experiments. It can activate TREM2 signaling pathways, making it a valuable tool for studying microglial activation and other TREM2-related functions. We recommend optimizing the concentration and conditions specific to your experimental setup.
What controls should I use when performing flow cytometry with this antibody?
When performing flow cytometry with the anti-TREM2 antibody, we recommend using an isotype control to assess non-specific binding. Additionally, including a known TREM2-negative cell line as a negative control and a TREM2-positive line as a positive control will help validate your results. These controls will ensure the specificity and accuracy of your staining.
Can this antibody be used to quantify TREM2 expression levels?
Yes, the anti-TREM2 antibody can be used to quantify TREM2 expression levels, particularly in Western blotting and flow cytometry. In Western blotting, the intensity of the bands can be quantified using densitometry. In flow cytometry, mean fluorescence intensity (MFI) can be used to assess relative expression levels. Both methods provide quantitative insights into TREM2 expression.
For Research Use Only. Not For Clinical Use.
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