Mouse Anti-HMGB1 Monoclonal Antibody (CBP6712)

Description

The high mobility histones were originally named because they are rich in content, relatively low in molecular weight, and exhibit "high mobility." In other words, they run very fast on SDS-PAGE gels. High mobility histone box 1 (HMGB1) is one of them. The "box" in the name refers to the so-called high mobility group frame, which is a compact domain involved in DNA binding and protein-protein interaction. The HMGB1 molecule has two such HMG domains (1). The protein is also called amphotericin. The name comes from the presence of two highly charged regions in the molecule, a relatively neutrally charged N-terminal and a very negatively charged C-terminal. The entire molecule is very unusually charged. The human sequence consists of 16.7% glutamic acid, 9.3% aspartic acid, 20% lysine, and 9.3% arginine. HMGB1 can bind toll-like receptors 2, 4, and 9 (TLR2, TLR4, and TLR9) and the receptor for advanced glycation end products (RAGE) (3,4).

Immunogen

Human full-length recombinant human HMGB1 protein expressed and purified in E. coli.

Species Reactivity

Human; Rat; Mouse

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2b

Clone Number

CBP6712

Applications

WB; IF; IHC

Application Notes

WB: 1:1000-1:2000
ICC/IF and IHC: 1:1000

Form

Liquid

Formulation

50% PBS, 50% glycerol

Preservatives

5mM NaN3

Concentration

1 mg/ml

Shipping

Gel Pack

Storage

Stable at 4°C for one year, for longer term store at -20°C

Handling Advice

The antibody solution should be gently mixed before use.

Research Use Only

For research use only

Target

HMGB1

Official Name

HMGB1

Full Name

High-mobility group box 1

Alternative Names

HMGB1; High-mobility group box 1

Gene ID

3146(Human); 100862258(Mouse); 25459(Rat)

Uniprot ID

P09429(Human); P63158(Mouse); P63159(Rat)