NeuroMab™ Mouse Anti-ITGAM Monoclonal Antibody (CBP1537)

Description

Mouse anti-growth cone antibody recognizes a protein originally called 2G13P, which is located on the growth cone. Subsequent studies determined that the protein was 40S ribosomal protein SA, also known as 37 kDa laminin receptor precursor or laminin receptor 1. 40S ribosomal protein SA is a membrane of 296 amino acids ~ 37 kDa, which is assembled And/or the required cytoplasmic and nuclear proteins. Or the stability of the 40S ribosomal subunit. In vertebrate evolution, this molecule has acquired a second function as a laminin receptor. It is expressed in cone cells especially in the filamentous feet and plate seborrheic of the developing rat central nervous system and embryonic neurons in culture. The 40S ribosomal protein SA interacts with the filamentous actin cytoskeleton and may therefore participate in the movement of growth cones. The mouse anti-growth cone antibody has been used to detect growth cones by immunohistochemistry and to identify 40S ribosomal protein SA in chicken and rat samples by Western blotting.

Immunogen

Embryonic chick tectal membranes.

Species Reactivity

Chicken; Mouse; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgM

Clone Number

CBP1537

Applications

IHC-P; IHC-Fr

Research Areas

Growth and Development

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1341

Form

Liquid

Formulation

Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
0.09% Sodium Azide

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

CD11b

Official Name

ITGAM

Full Name

Integrin alpha M

Alternative Names

ITGAM; CD11B; CR3A; MAC-1; MAC1A; MO1A; SLEB6; integrin subunit alpha M

Gene ID

3684 (Human); 16409 (Mouse); 25021 (Rat)

Uniprot ID

P11215 (Human); P05555 (Mouse)

1. Stettler, O. et al. (1999) Monoclonal antibody 2G13, a new axonal growth cone marker. 2. Baloui, H. et al. (2004) Cellular prion protein/laminin receptor: distribution in adult central nervous system and characterization of an isoform associated with a subtype of cortical neurons. 3. Espejo, C. et al. (2005) Time-course expression of CNS inflammatory, neurodegenerative tissue repair markers and metallothioneins during experimental autoimmune encephalomyelitis. 4. Penkowa, M. et al. (2003) Metallothionein-I overexpression alters brain inflammation and stimulates brain repair in transgenic mice with astrocyte-targeted interleukin-6 expression. 5. Kim, S.R. et al. (2011) Dopaminergic pathway reconstruction by Akt/Rheb-induced axon regeneration. 6. Nordman, J.C. & Kabbani, N. (2012) An interaction between α7 nicotinic receptors and a G-protein pathway complex regulates neurite growth in neural cells