NeuroMab™ Rat Anti-Itgam Monoclonal Antibody (CBP1596)

Product Overview

Description

Rat anti-Mouse CD11b antibody recognizes murine CD11b cell surface antigen, also known as αM integrin chain or MAC-1, which is a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.
The expression of CD11b increases during the maturation of monocytes, and the expression level is different on tissue macrophages. According to reports, peritoneal macrophages express higher levels of CD11b than splenic macrophages.
According to reports, rat anti-mouse CD11b antibodies can block the binding of iC3b to its receptor (Beller et al. 1982).
It is reported that rat anti-mouse CD11b antibody is suitable for PLP-fixed paraffin-embedded tissue, but it has not been tested for formalin-fixed tissue.

Immunogen

T cell enriched splenocytes from B10 mice.

Species Reactivity

Mouse; Human; Rabbit

Clonality

Monoclonal

Host Species

Rat

Isotype

IgG2b

Clone Number

CBP1596

Applications

Application Notes

FCM (1/10)

Relevant Diseases

Alzheimer's Disease

Research Areas

Growth and Development

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1377

Product Properties

Form

Liquid

Formulation

PBS
0.09% Sodium Azide
1% BSA

Concentration

0.05 mg/ml

Purification

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Target

CD11b

Official Name

Itgam

Full Name

Integrin alpha M

Alternative Names

ITGAM; CD11B; CR3A; MAC-1; MAC1A; MO1A; SLEB6; integrin subunit alpha M

Gene ID

3684 (Human); 16409 (Mouse); 25021 (Rat)

Uniprot ID

P11215 (Human); P05555 (Mouse)

References

1. Terrando, N. et al. (2010) The impact of IL-1 modulation on the development of lipopolysaccharide-induced cognitive dysfunction.
2. Redensek, A. et al. (2011) Expression and detrimental role of hematopoietic prostaglandin D synthase in spinal cord contusion injury.
3. Brochard, V. et al (2009) Infiltration of CD4+ lymphocytes into the brain contributes to neurodegeneration in a mouse model of Parkinson disease.
4. Chinnery, H.R. et al. (2010) Novel characterization of monocyte-derived cell populations in the meninges and choroid plexus and their rates of replenishment in bone marrow chimeric mice.
5. Ferger, A.I. et al (2010) Effects of mitochondrial dysfunction on the immunological properties of microglia.

Publications