NeuroMab™ Mouse Anti-ITGAM Monoclonal Antibody (CBP1533)

Description

Mouse anti-rat neurofilament 200kDa antibody, clone RT97 recognizes 200kDa neurofilament protein in multiple species. Mouse anti-rat Neurofilament 200kDa antibody, clone RT97 stains a variety of tumors, including pheochromocytoma, paraganglioma and ganglion neuroblastoma. Mouse anti-rat Neurofilament 200kDa antibody, clone RT97 also recognizes fetal tau by Western blotting, Alzheimer's paired helical filament tau and phosphorylation-dependent epitopes on microtubule-associated protein 1B (MAP1B), but Similar reactivity is not obvious in immunohistochemistry research.

Immunogen

Triton X-100-insoluble rat brain protein.

Species Reactivity

Rat; Mouse; Reptile; Human; Chicken; Porcine

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP1533

Applications

IHC-P; IHC-Fr

Application Notes

IHC-P (1/50-1/100)

Research Areas

Growth and Development

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1338

Form

Liquid

Formulation

PBS
0.1% Sodium Azide
0.1% BSA

Concentration

0.1 mg/ml

Purification

Purified IgG prepared by hydrophobic interaction chromatography from tissue culture supernatant.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

CD11b

Official Name

ITGAM

Full Name

Integrin alpha M

Alternative Names

ITGAM; CD11B; CR3A; MAC-1; MAC1A; MO1A; SLEB6; integrin subunit alpha M

Gene ID

3684 (Human); 16409 (Mouse); 25021 (Rat)

Uniprot ID

P11215 (Human); P05555 (Mouse)

1. Weber, K. et al. (1983) Neurofilaments, a subclass of intermediate filaments: structure and expression. 2. Anderton, B.H. et al. (1982) Monoclonal antibodies show that neurofibrillary tangles and neurofilaments share antigenic determinants. 3. Johnstone, M. et al. (1997) The neurofilament antibody RT97 recognises a developmentally regulated phosphorylation epitope on microtubule-associated protein 1B. 4. Tonge, D.A. et al. (1996) Expression of a developmentally regulated, phosphorylated isoform of microtubule-associated protein 1B in sprouting and regenerating axons in vitro. 5. Sann, H. et al. (1995) RT97: a marker for capsaicin-insensitive sensory endings in the rat skin. 6. Veeranna, et al. (2008) Neurofilament tail phosphorylation: identity of the RT-97 phosphoepitope and regulation in neurons by cross-talk among proline-directed kinases. 7. Logan, A. et al. (2006) Neurotrophic factor synergy is required for neuronal survival and disinhibited axon regeneration after CNS injury. 8. Lorber, B. et al. (2004) Stimulated regeneration of the crushed adult rat optic nerve correlates with attenuated expression of the protein tyrosine phosphatases RPTPalpha, STEP, and LAR. 9. Reynolds, J. et al. (2005) Age-dependent changes in Fibroblast growth factor 2 (FGF-2) expression in mouse cerebellar neurons. 10. Shin, D.H. et al. (2003) The correspondence between the labeling patterns of antibody RT97, neurofilaments, microtubule associated protein 1B and tau varies with cell types and development stages of chicken retina. 11. Connolly,A.A. et al. (1987) A comparative study of a silver stain and monoclonal antibody reactions on Alzheimer's neurofibrillary tangles. 12. Doering, L.C. (1991) Transplantation of fetal CNS tissue into the peripheral nervous system: a model to study aberrant changes in the neuronal cytoskeleton. 13. McCarthy, P.W. et al. (1992) RT97- and calcitonin gene-related peptide-like immunoreactivity in lumbar intervertebral discs and adjacent tissue from the rat. 14. Murphy, A. et al. (1993) Neurofilament expression in human T lymphocytes. 15. Yabe, J.T. et al. (2001) Neurofilaments consist of distinct populations that can be distinguished by C-terminal phosphorylation, bundling, and axonal transport rate in growing axonal neurites. 16. Wang, S. et al. (2000) Progressive optic axon dystrophy and vacuslar changes in rd mice. 17. Kuwamura, M. et al. (2004) Cerebral ganglioneuroblastoma in a golden retriever dog. 18. Rovere, G. et al. (2015) Comparison of Retinal Nerve Fiber Layer Thinning and Retinal Ganglion Cell Loss After Optic Nerve Transection in Adult Albino Rats. 19. Wang, J. et al. (2017) MicroRNA regulation in an animal model of acute ocular hypertension. 20. Vidal-Sanz, M. et al. (2015) Retinal neurodegeneration in experimental glaucoma.