NeuroMab™ Anti-CD54 Antibody(NRP-0422-P1408)

Figure 1 Binding of seven unique scFv clones to Ramos cells (solid bars) and Jurkat cells (open bars). Control scFv (ctrl) did not bind to any cells.

Figure 2 Anti-Ramos scFv induces apoptosis.

Seven unique scFv clones were incubated with Ramos B lymphoma cells at different concentrations for 24 hours at 37°C, and the effect on apoptosis induction was studied. Three scFvs; Bl, B11 and C11 showed titratable activity against both cell lines, whereas apoptosis-inducing

Figure 3 Specificity of isolated antibodies including HLA-DR/DP, IgM and ICAM-1.

50-600 x 106 Raji B lymphoma cells were lysed with 0.5% v/v of the non-ionic detergent Triton X100TM and immunoprecipitated with 100 μg of B1 (lane 1) and B11 (lane 2) antibodies in fully human IgG1 format, Then cross-linked with Protein A SepharoseTM. Ramos B lymphoma cell lysate from 50 x 106 cells was used to pellet 20 μg C11 (lane 3).

Figure 4 Specificity of isolated antibodies including HLA-DR/DP, IgM and ICAM-1.

Binding of B1 IgG, B11 IgG and Cll IgG to B lymphoma cells was specifically blocked by preincubation with anti-HLA-DR/DP, anti-ICAM-1 or anti-IgM antibodies, respectively.

Figure 5 Binding of B1, B11, C11 IgG1 to tumor cell lines of different origins. The antigen distribution of the antigens targeted by the B1, B11 and C11 antibodies on different cancer cell lines was studied by flow cytometry.