Immortalized Neuron Model Construction Service

Neurons are the fundamental units of the nervous system (NS), responsible for transmitting information throughout the body. Unlike most cell types, neurons typically permanently lose their proliferative ability after differentiation, and neurons in the adult NS generally remain in a quiescent state. The capability to proliferate indefinitely makes immortalized neurons a valuable tool for studying neurotoxicity and modeling neurological diseases.

Creative Biolabs has extensive experience in neurotoxicity studies and modeling Alzheimer's Disease (AD) and Parkinson's Disease (PD) using immortalized neurons. Please contact us to discuss details about your project.

Popular Neuron Models

The following neuron cell line models are widely used for research:

Human-derived Neurons

• SH-SY5Y Human Neuroblastoma Cell Line
• Immortalized Human Neural Progenitor Cell Line

Mouse-derived Neurons

• HT22 Hippocampal Neuronal Cell Line
• Mouse Neuro-2A Cell Line (N2a)
• Mouse Midbrain Dopaminergic Neuron Cell MN9D

Rat-derived Neurons

• PC12 Rat Pheochromocytoma Cell Line
• N27 Rat Dopaminergic Neural Cell Line

Case Study

The SH-SY5Y cell line is an essential cellular model in neuroscience research, widely used for the study of AD and PD. The cell line can be differentiated through various induction protocols to simulate neuronal characteristics, thereby providing an effective experimental platform for disease modeling and drug screening.

1. Disease modeling

Modeling AD: Researchers can add exogenous amyloid-beta (Aβ1-42 and Aβ1-40), or oxidative stress inducers such as H2O2 to the WT cells to induce disease phenotypes.

Mimicking PD: In the study of PD, pathological changes in SH-SY5Y cells can be induced using MPP+, 6-OHDA, or rotenone. These factors are closely associated with the death of dopaminergic neurons and the aggregation of alpha-synuclein.

Fig. 1 AD in vitro model of Aβ1-42-induced neuronal injury.¹

2. Drug Screening

Drug screening experiments often use the SH-SY5Y cell line to evaluate the potential effects of a drug candidate on neuronal function. Researchers can perform cell viability assay, apoptosis assay, neurite outgrowth assay, mitochondrial function assay, oxidative stress assay, and neuroinflammatory assay to screen for potential neuroprotective or neurotoxic compounds.

Fig. 2 miR-6076 inhibition reduces Aβ1-42-induced neuronal damage in SH-SY5Y cells.¹

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Reference

1. Lin, Yujian et al. "miR-6076 targets BCL6 in SH-SY5Y cells to regulate amyloid-β-induced neuronal damage." J Cell Mol Med. 2023;27(24):4145-4154. Distributed under Open Access license CC BY 4.0. The original images were modified.