Immortalized Oligodendrocyte Modeling Service

Oligodendrocytes are crucial glial cells in the central nervous system (CNS) responsible for the formation and maintenance of the myelin sheath, which insulates axons and enhances the speed of electrical signal conduction. They play a vital role in maintaining the overall health and functionality of neurons, and their ability to support neuronal survival and promote signal transmission is essential for normal brain function.

Since oligodendrocytes are present in small numbers in the CNS and cannot proliferate, and there is a lack of effective separation and purification techniques, the isolation of primary oligodendrocytes has always posed significant challenges. Creative Biolabs is proud to use immortalized oligodendrocytes, which are valuable tools, to help scientists explore oligodendrocyte differentiation and facilitate the development of potential therapeutic strategies for conditions such as multiple sclerosis and spinal cord injuries.

Popular Oligodendrocyte Cell Lines

The following oligodendrocyte cell lines are widely used for research:

Human-derived Oligodendrocytes

• Human Glial (Oligodendrocytic) Hybrid Cell Line (MO3.13)

Mouse-derived Oligodendrocytes

• Mouse Immortalized Oligodendrocytes from Creative Biolabs

Rat-derived Oligodendrocytes

• Rat Oligodendroglia Cell Line OLN-93
• Rat Immortalized Oligodendrocytes from Creative Biolabs

Case Study

Human Glial (Oligodendrocytic) Hybrid Cell Line, MO3.13 exhibits differential gene expression profiles that support the characteristics of oligodendrocytes and astrocytes. It can differentiate into an oligodendrocyte phenotype under specific conditions and is widely used to study oligodendrocyte differentiation and neurodegenerative diseases.

• Drug Toxicity Testing

Varying concentrations of Cuprizone-induced cytotoxicity were assessed in the MO3.13 cell line via the MTT assay. Results indicated a significant reduction in cell viability across all cuprizone-treated groups after this exposure period. In contrast, previously tested antipsychotics, Cannabidiol, and Benztropine demonstrated no cytotoxic effects on the MO3.13 cell line when evaluated concurrently.

Fig. 1 Cuprizone-induced cytotoxicity was measured in MO3.13 cultures via MTT assay.¹

2. Oligodendrocytes Differentiation

To explore the influence of D‐Asp on oligodendrocyte differentiation, human oligodendrocyte precursor cells (MO3.13) were treated with D‐Asp. Subsequently, the expression levels of 2',3'‐cyclic nucleotide 3'‐phosphodiesterase (CNPase), and myelin basic protein (MBP) were assessed via RT–PCR and Western blotting.

Fig. 2 Effects of D-Asp exposure on oligodendrocytes differentiation.²

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Elevate your research with our Immortalized Oligodendrocyte Model Service! Our dedicated Neuros Team is available to assist you with any questions or inquiries, ensuring you receive the support you need for your scientific endeavors. Reach out to us today for expert guidance!

References

1. Falvella, Ana Caroline Brambilla et al. "Cannabidiol Displays Proteomic Similarities to Antipsychotics in Cuprizone-Exposed Human Oligodendrocytic Cell Line MO3.13." Front Mol Neurosci. 2021 Nov 30;14:814907. Distributed under Open Access license CC BY 4.0 without modification.
2. de Rosa, Valeria et al. "D-Aspartate treatment attenuates myelin damage and stimulates myelin repair." EMBO Mol Med. 2019;11(1):e9278. Distributed under Open Access license CC BY 4.0. The original image was modified.