Peripheral Neuroinflammation Drug Discovery Service

Creative Biolabs is devoted to providing a variety of services in the field of life science to global customers. Neuroscience is a growing field with many cutting-edge scientific challenges that need to be addressed. Our experts in neuroscience integrate resources worldwide to help researchers design customized one-stop solutions, which enhance the mechanism of action studies and drug research and development in peripheral neuroinflammation.

Background of Peripheral Neuroinflammation

Chronic pain is a health problem that affects a large amount of the population. Chronic pain is generally considered to be associated with a heightened perception of pain caused by the sensitization of peripheral primary sensory neurons and central nociceptive neurons. Peripheral neuroinflammation occurs in the peripheral nerves and ganglia, which is intimately related to the pain. Creative Biolabs assists in understanding the mechanisms of action (MoAs) of peripheral neuroinflammation-caused chronic pain, which may contribute to healing those suffering from chronic pain.

Mechanism Studies of Peripheral Neuroinflammation

The impairments and infection lead to peripheral inflammation. The immune cells such as macrophages and mast cells start to release inflammatory factors, such as nerve growth factors (NGF), bradykinin, prostaglandins, H⁺, adenosine triphosphate (ATP), prostaglandins, as well as pro-inflammatory cytokines and chemokines, which directly irritate the peripheral pain-sensing nociceptor nerve fibers located in peripheral tissues via G protein-coupled receptors (GPCRs), ionotropic receptors, and tyrosine kinase receptors. The activation triggers second messengers that stimulate a range of kinases such as PKA, PKC, MAPK, and ERK, which lead to peripheral sensitization with hypersensitivity and hyperexcitability of nociceptor neurons. In addition, nociceptor neurons could also be stimulated by endogenous ligands, such as microRNAs, and exogenous ligands, such as bacterial components, via toll-like receptors (TLRs) on the peripheral nociceptor nerve fibers. The long-term peripheral neuroinflammation leads to alterations in neurotransmitter transcription and ultimately causes chronic pain.

Fig.1 Inflammation elicits pain via inflammatory mediators and peripheral sensitization. (Ji, 2014)

Peripheral Neuroinflammation Solutions at Creative Biolabs

There is an urgent need to understand the mechanism of pain caused by peripheral neuroinflammation for the investigation of innovative targeting therapeutics. For this purpose, Creative Biolabs is committed to offering one-stop solutions for peripheral neuroinflammation research and development. We have a variety of in vitro, in vivo, and ex vivo assays with neuron cultures and animal models, along with our sophisticated imaging and electrophysiology technologies. We offer a wide range of neuroinflammation assays. Cytokine release assay enables to test inflammatory factors, pro-inflammatory cytokines, and chemokines released by macrophages and mast cells during peripheral neuroinflammation. In addition, our platform is able to perform lipopolysaccharide (LPS)-induced inflammation assay and nitric oxide production assay to investigate peripheral neuroinflammation. Furthermore,we provide neurotransmitter detection assays to detect the alterations of multiple neurotransmitters in peripheral neuroinflammation. Our customized one-stop solution enables to expore the mechanisms of action study in peripheral neuroinflammation and develop targeting therapeutics. More details about our platform and services for translational research and integrated research in peripheral neuroinflammation are below.

If you have the intention to customize your preclinical peripheral neuroinflammation drug discovery project, please feel free to contact us for more information.

Reference

1. Ji, R. R.; et al. Emerging targets in neuroinflammation-driven chronic pain. Nature reviews Drug discovery. 2014, 13 (7): 533-548.