Custom hiPSC Differentiation Service

Human induced pluripotent stem cells (hiPSCs) have become a valuable tool for research, drug development, and regenerative medicine due to their ability to differentiate into various cell types. Creative Biolabs employs advanced technology to support scientists to have access to high-quality differentiated cells for their disease modeling and drug screening.

Our Workflow

The workflow for hiPSC differentiation service typically involves several key steps to guide the differentiation of hiPSCs into desired cell types for research use. While specific protocols may vary depending on the service provider and the target cell type, our general workflow can be outlined as follows:

Available Cell Lineages

We accommodate a diverse range of cell lineages, including neurons, astrocytes, and microglia among others. Below is a partial list of the lineages we provide. If you don't find a specific lineage you're looking for, we also offer tailored development of differentiation protocols for any unlisted lineages.

Case Study

Researchers used iPSCs from Researchers used iPSCs from Alzheimer's disease (AD) patients with different genetic backgrounds, edited them to create isogenic controls, and differentiated them into various cell types of the brain, such as neurons, oligodendrocytes, microglia, astrocytes, pericytes, and brain microvascular endothelial cells. These cells were cultured in 2D monoculture or 3D coculture, which greatly promoted the study of the role of APOE in the occurrence and development of AD.

Fig. 1 Employing hiPSC-derived cells and 3D model to dissect the role of APOE in AD.¹

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Reference

1. Pinals, Rebecca L, and Li-Huei Tsai. "Building in vitro models of the brain to understand the role of APOE in Alzheimer's disease." Sci Alliance. 2022 Dec 1;6(2):e202201845. Distributed under Open Access license CC BY 4.0 without modification.