Custom hiPSC Differentiation Service

Human induced pluripotent stem cells (hiPSCs) have become a valuable tool for research, drug development, and regenerative medicine due to their ability to differentiate into various cell types. Creative Biolabs employs advanced technology to support scientists to have access to high-quality differentiated cells for their disease modeling and drug screening.

Workflow Available Cell Lineages Case Study Related Services

Our Workflow

The workflow for hiPSC differentiation service typically involves several key steps to guide the differentiation of hiPSCs into desired cell types for research use. While specific protocols may vary depending on the service provider and the target cell type, our general workflow can be outlined as follows:

Step 1	Consultation and Project Design Initial Consultation: Discuss objectives, cell types of interest, and desired deliverables with the service team. Project Customization: Define the specific requirements of the project, including any quality controls, and timelines.
Step 2	hiPSC Line Selection and Preparation Choosing a hiPSC Line: Select from customer cell lines, our control iPSC lines and genetically engineered iPSCs that meet the project needs or use a custom line. hiPSC Validation, Expansion, Adaptation: Validate the pluripotency of hiPSCs, expand the validated cells for sufficient quantity, and adapt them to specific culture conditions for successful differentiation into target cell types.
Step 3	hiPSC Differentiation Protocol Development: Select or design a differentiation protocol tailored to the desired lineage (e.g., neurons, astrocytes, microglia). Adjust parameters based on specific needs, including growth factors and small molecules for directed differentiation. Differentiation: Initiate differentiation under controlled conditions using appropriate media and supplements. Regularly monitor cell morphology, growth, and expression of pluripotency and lineage-specific markers through microscopy and assays.
Step 4	Cell Characterization Phenotypic Assessment: We perform rigorous quality control checks to confirm that differentiated neural cells are free from contaminants and express specific lineage markers with high viability post-thaw. Function Testing: For detailed functional characterization, we provide optional assays like microelectrode arrays, neurite outgrowth assays, and calcium transient assays to evaluate neuronal activity and functionality.

Available Cell Lineages

We accommodate a diverse range of cell lineages, including neurons, astrocytes, and microglia among others. Below is a partial list of the lineages we provide. If you don't find a specific lineage you're looking for, we also offer tailored development of differentiation protocols for any unlisted lineages.

Neural Stem Cells	Neural Precursor Cells	Astrocytes	Microglia
Oligodendrocytes	Cortical Neurons	Striatal Neurons	Peripheral Neurons
Glutamatergic Neurons	GABAergic Neurons	Dopaminergic Neurons	Cholinergic Neurons
Motor Neurons	Sensory Neurons	Brain Microvascular Endothelial Cells
Pericytes	Skeletal Muscle Cells	Smooth Muscle Cells	Schwann Cells
Retinal Pigment Epithelial Cells		Monocytes	Macrophages
Cardiomyocytes	Hepatocytes	Photoreceptor Cells	Kidney Cells

Case Study

Researchers used iPSCs from Researchers used iPSCs from Alzheimer's disease (AD) patients with different genetic backgrounds, edited them to create isogenic controls, and differentiated them into various cell types of the brain, such as neurons, oligodendrocytes, microglia, astrocytes, pericytes, and brain microvascular endothelial cells. These cells were cultured in 2D monoculture or 3D coculture, which greatly promoted the study of the role of APOE in the occurrence and development of AD.

Fig 1. Employing hiPSC–based cellular and tissue models to deconvolute the function of APOE in AD. Fig. 1 Employing hiPSC-derived cells and 3D model to dissect the role of APOE in AD.¹

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Reference

Pinals, Rebecca L, and Li-Huei Tsai. "Building in vitro models of the brain to understand the role of APOE in Alzheimer's disease." Sci Alliance. 2022 Dec 1;6(2):e202201845. Distributed under Open Access license CC BY 4.0 without modification.

For Research Use Only. Not For Clinical Use.

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