NeuroMab™ Anti-GARP Antibody(NRP-0422-P1641)

Product Overview

Description

The antibody inhibits human Treg function in in vitro suppression assays. The antibody binding to GARP in the presence of TGF-β, cross-reactive with cynomolgus GARP-TGFβ1.

Species Reactivity

Human; Cynomolgus Monkey

Clonality

Monoclonal

Host Species

Humanized

Applications

FC; Inhib; Block; In Vitro; In Vivo

Relevant Diseases

Amyotrophic Lateral Sclerosis

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

> 95% (SDS-PAGE)

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

GARP

Official Name

LRRC32

Full Name

Leucine Rich Repeat Containing 32

Alternative Names

Leucine Rich Repeat Containing 32; Glycoprotein A Repetitions Predominant; Transforming Growth Factor Beta Activator LRRC3; Leucine-Rich Repeat-Containing Protein 3; D11S833E; Garpin; GARP; LRRC32

Gene ID

2615(Human); 434215(Mouse)

Uniprot ID

Q14392(Human)

Product Pictures

Inhib

Figure 3 MHGARP8 and LHG-10 inhibit human Treg from producing active TGF-β.

After a short in vitro amplification, human CD4+CD25hiCD127lo cells (Tregs) were re-stimulated with anti-CD3/CD28 coated beads during 24 hours, in the presence or absence of the indicated mAbs (10 μg/ml). Cells lysates were analyzed by Western Blot with antibodies against phosphorylated SMAD2 (pSMAD2), as a read-out for active TGF-β production, or β-ACTIN (loading control).

FCM

Figure 1 Cross-reactivity with macaque GARP-TGFβ measured on cells by FACS. 293E cells were transfected with human/cynomolgus GARP and human/cynomolgus TGFB. LHG-10-D and affinity-optimized variants cross-react with cynomolgus GARP-TGFB.