iNeuMab™ Anti-Tau Antibody (NRP-0422-P2275)

Q: What are the recommended dilution ratios for different applications?

For Western blotting, a typical dilution range of 1:1000 to 1:2000 is recommended. For immunohistochemistry and immunofluorescence, a dilution of 1:200 to 1:500 is suggested, depending on the tissue type and antigen retrieval methods used. It is advisable to optimize the dilution for your specific experimental conditions for best results.

Q: Can this antibody be used for detecting phosphorylated Tau?

Although the antibody primarily targets non-phosphorylated Tau, it does recognize certain phosphorylated forms. However, for specific studies focusing exclusively on phosphorylated Tau, using an antibody specifically designed for phosphorylated epitopes may provide more accurate results. It’s crucial to review experimental requirements before selection.

Q: Are there any known non-specific bindings or cross-reactivities associated with this antibody?

The antibody has been rigorously tested for specificity and is known to have minimal non-specific bindings or cross-reactivities. However, as with any antibody, it is essential to include appropriate controls in your experiments to ensure the validity of your results. Testing in your specific experimental conditions is recommended.

Q: What support is available if I encounter issues with the antibody in my experiments?

If you encounter any issues with the antibody, our technical support team is readily available to assist you. We offer comprehensive troubleshooting guides, detailed product datasheets, and personalized support to help you optimize your experiments and resolve any challenges. Our goal is to ensure that you achieve the best possible results with our antibody.

Product Overview

Description

NRP-0422-P2275 is an IgM-class monoclonal antibody that stains the fibrillar pathology (dystrophic neurites, neurofibrillary tangles, and neuropil threads) commonly observed in postmortem histological analysis of Alzheimer's disease (AD)1 brain. NRP-0422-P2275 reacts with tau and that its epitope is located at the N terminus (11-13) of human tau.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgM

Applications

WB; ELISA; In Vitro; In Vivo

Relevant Diseases

Alzheimer's Disease

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

Tau

Official Name

Tau

Alternative Names

MAPT; DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU; microtubule associated protein tau; Tau proteins; tau-40

Publications

Publications (0)

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Neural Genes

Lenti of Human microtubule-associated protein tau (MAPT) transcript variant (NM_016835) ORF clone, mGFP Tagged

Lenti of Human microtubule-associated protein tau (MAPT) transcript variant (NM_016841) ORF clone, mGFP Tagged

Lenti of Human microtubule-associated protein tau (MAPT) transcript variant (NM_001123067) ORF clone, Myc-DDK Tagged

Lenti of Human microtubule-associated protein tau (MAPT) transcript variant 5 (NM_001123067) ORF clone, mGFP Tagged

Lenti of Human microtubule-associated protein tau (MAPT) transcript variant 4 (NM_016841) ORF clone, mGFP Tagged

Lenti of Human microtubule-associated protein tau (MAPT) transcript variant (NM_001123066) ORF clone, Myc-DDK Tagged

Lenti of Human microtubule-associated protein tau (MAPT) transcript variant (NM_016835) ORF clone, Myc-DDK Tagged

Lenti of Mouse microtubule-associated protein tau (Mapt) transcript variant 2 (NM_010838) ORF clone, mGFP Tagged

Lenti of Human microtubule-associated protein tau (MAPT) transcript variant 2 (NM_005910) ORF clone, Myc-DDK Tagged

Human microtubule-associated protein tau (MAPT) transcript variant 4 (NM_016841) ORF clone, Untagged

Neural Antibodies

Anti-Tau Antibody (CBP17521)

Mouse Anti-MAPT Monoclonal Antibody (CBP4065)

iNeuMab™ Mouse Anti-Tau 189-195 Monoclonal Antibody (CBP2495)

iNeuMab™ Anti-TAU BBB Shuttle Antibody (NRZP-1222-ZP1183)

iNeuMab™ Mouse Anti-Tau 189-195 Monoclonal Antibody (CBP2494)

iNeuMab™ Anti-Tau BBB Shuttle Antibody (NRZP-1222-ZP1175)

Mouse Anti-Human Tau Monoclonal Antibody (CBP2505)

Mouse Anti-MAPT Monoclonal Antibody (CBP2789)

Anti-Tau Antibody (CBP17523)

Anti-Tau Antibody (CBP17522)

Scientific Resources

[Brochure]

Neuros Antibody Product Panel

View

[Brochure]

Histology & Immunohistochemistry Services

View

[Brochure]

In Vitro Service

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

A must-have for tauopathy research

It has provided us with consistent results in both Western blotting and immunofluorescence assays. The antibody's high specificity minimizes background noise, enhancing our data clarity.

Excellent

The results have been consistent and reliable

We switched to the antibody, after experiencing issues with other antibodies. The difference was remarkable. The antibody's performance in our ELISA assays has been outstanding, giving us reliable and reproducible data.

Excellent

It's definitely worth the investment

The high specificity and low background staining of the antibody, make it an excellent choice for immunohistochemistry. It has significantly improved the quality of our staining, making it easier to interpret our results.

Excellent

Highly recommended

After trying multiple tau antibodies, we found the NeuroMab™ Anti-Tau Antibody, Clone NR2218P, to be the most reliable. Its high specificity and sensitivity have made a significant difference in our research on neurodegenerative diseases.

Excellent

The antibody's high specificity is particularly noteworthy

The performance of the antibody in our experiments has been exceptional. The antibody's ability to detect tau protein with high specificity has greatly enhanced our research on tauopathies. It's a top-notch product.

Q&As

What are the recommended dilution ratios for different applications?

For Western blotting, a typical dilution range of 1:1000 to 1:2000 is recommended. For immunohistochemistry and immunofluorescence, a dilution of 1:200 to 1:500 is suggested, depending on the tissue type and antigen retrieval methods used. It is advisable to optimize the dilution for your specific experimental conditions for best results.

Can this antibody be used for detecting phosphorylated Tau?

Although the antibody primarily targets non-phosphorylated Tau, it does recognize certain phosphorylated forms. However, for specific studies focusing exclusively on phosphorylated Tau, using an antibody specifically designed for phosphorylated epitopes may provide more accurate results. It’s crucial to review experimental requirements before selection.

Are there any known non-specific bindings or cross-reactivities associated with this antibody?

The antibody has been rigorously tested for specificity and is known to have minimal non-specific bindings or cross-reactivities. However, as with any antibody, it is essential to include appropriate controls in your experiments to ensure the validity of your results. Testing in your specific experimental conditions is recommended.

What support is available if I encounter issues with the antibody in my experiments?

If you encounter any issues with the antibody, our technical support team is readily available to assist you. We offer comprehensive troubleshooting guides, detailed product datasheets, and personalized support to help you optimize your experiments and resolve any challenges. Our goal is to ensure that you achieve the best possible results with our antibody.