- Host Species:
- Mouse
- Species Reactivity:
- Human
- Applications:
- WB; ELISA; ICC; IHC
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
SPECIFIC INQUIRY
inquiryProduct Overview
Description
NRP-0422-P1668 antibody recognizes all tau isoforms. NR2264P produces abundant staining of NFT, dystrophic neurites in plaques, and dispersed staining of neuropil (neuropil threads). The monoclonal antibodies can be used to detect tau and abnormally phosphorylated tau in brain extracts and in unconcentrated cerebrospinal fluid.
Species Reactivity
Human
Clonality
Monoclonal
Host Species
Mouse
Isotype
IgG1
Applications
WB; ELISA; ICC; IHC
Relevant Diseases
Alzheimer's Disease
Product Properties
Formulation
PBS only
Preservatives
BSA Free
Concentration
1mg/mL
Purification
Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
Purity
> 95% (SDS-PAGE)
Endotoxin Level
Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg
Low Endotoxin < 1 EU/mg
Shipping
Gel Packs
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Research Use Only
For research use only
Target
Product Pictures
WB
Figure 1 Western blot detection of normal tau or PHF-tau using monoclonal antibodies Tau-1 and AT120.
Lanes 1, 3 and 5: PHF-tau isolated according to Greenberg and Davies (1990). Lanes 2, 4 and 6: Normal affinity purified human tau according to Mercken et al. (1992a). Lane 7: Molecular weight markers. Lanes 1 and 2 were developed using AT8, lanes 3 and 4, using AT120 and lanes 5 and 6, using Tau-1 monoclonal antibody.
ELISA
Figure 2 Titration of normal (0) and PHF tau (▪) spiked into a pool of tau-negative CSF using an amplified (CARD) AT120 sandwich ELISA. All dilutions were tested in duplicate and data are expressed in optical density (OD) units.
WB
Figure 3 Western blot of several deletion mutants stained with AT120.
Publications
Publications (0)
Kits
Neural Antibodies
Neural Genes
Neural Cell Lines
Scientific Resources
Customer Reviews and Q&As
Customer Reviews
Average Customer Ratings
Overall
5.0
ExcellentOverall, a highly reliable antibody for neurodegenerative researchWe primarily use it for Western Blotting, and it consistently provides clear, specific bands with minimal background noise. The antibody's sensitivity is impressive, allowing us to detect even low levels of tau protein.
ExcellentI have used the antibody for Western Blot and ICC applicationsThe antibody provides excellent specificity and clarity in WB, producing distinct bands that are easy to interpret. For ICC, the antibody beautifully stains tau in cultured neurons, highlighting fine structural details without excessive background.
ExcellentIt provides strong, specific signals that are easy to quantifyThe antibody's robustness and reproducibility have significantly enhanced the quality of our data. Additionally, the customer support from Creative Biolabs has been exceptional, assisting us in troubleshooting and optimizing our protocols.
ExcellentOne of the best antibodies for detecting tau proteinThe antibody provides clear and specific staining of tau protein in cultured neurons, highlighting intricate cellular structures. Its high specificity ensures that our observations are accurate and reliable.
ExcellentA high-quality antibody that we highly recommendWe've been using the antibody in our Alzheimer's research, and it has proven to be an excellent choice. The antibody provides strong, specific signals in Western Blot and ELISA, allowing us to accurately measure tau levels.
Q&As
How specific is the antibody to different tau isoforms or phosphorylated states?
The Clone NR2264P is meticulously validated for its specificity to tau protein across its various isoforms. It recognizes both phosphorylated and non-phosphorylated forms, which is crucial for differentiating between normal tau function and pathological states. This antibody has undergone extensive testing to ensure minimal cross-reactivity with other proteins, making it an excellent tool for detailed tau analysis in your research.
Are there any published studies that have used this antibody, and can you provide examples?
Yes, numerous studies have utilized the antibody. For example, it has been employed in research examining tau pathology in Alzheimer's disease models, contributing to insights into disease mechanisms. Peer-reviewed articles often cite its use in characterizing tau distribution in brain tissues. For specific references, we recommend checking our website or contacting our support team for a list of relevant publications.
What is the delivery time for this antibody, and do you offer any customer support for experimental troubleshooting?
Typically, the antibody is shipped within 1-2 weeks after order placement, depending on stock availability. We prioritize efficient delivery to support your research timelines. Additionally, our customer support team is available to assist with any experimental troubleshooting or technical queries you may have. We provide comprehensive support to ensure your experiments with our products are successful and productive.
For Research Use Only. Not For Clinical Use.
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