Human Tau proteins ELISA kit
[CAT#: NRZP-0722-ZP220]
For the quantitative determination of human tau proteins concentrations in serum, plasma, cerebrospinal fluid (CSF).
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
This assay has high sensitivity and excellent specificity for detection of human Tau proteins.
Species Reactivity
Human
Relevant Diseases
Alzheimer's Disease
Marker
microtubule-associated protein tau
Properites
Sensitivity
In most cases, the sensitivity is below 3.9 pg/ml.
Detection Method
Sandwich
Sample Type
serum, plasma, cerebrospinal fluid (CSF)
Assay Type
quantitative
Assay Duration
1-5h
Number Of Samples
50-100ul
Detection Range
15.6 pg/ml-1000 pg/ml
Max Emission
450 nm
Target Details
Target
Tau
Official Name
MAPT
Full Name
microtubule associated protein tau
Alternative Names
DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU, G protein beta1/gamma2 subunit-interacting factor 1, microtubule-associated protein tau, isoform 4
Gene ID
4137(Human)
Uniprot ID
P10636(Human)
Protocols
Protocols
1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
Publications
Publications (0)
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Neural Antibodies
Neural Genes
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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