Human Tau proteins ELISA kit

Description

This assay has high sensitivity and excellent specificity for detection of human Tau proteins.

Species Reactivity

Human

Marker

microtubule-associated protein tau

Principle

The human Tau ELISA Kit is engineered for accurate measurement of human Tau levels from samples including serum, plasma, or cerebrospinal fluid (CSF). It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the Tau content in the sample. The color intensity is positively correlated with Tau content in the sample. The Tau concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.
Tau, also called MAPT, is the main component of neurofibrillary tangles (NFTs). As a stabilizing microtubule-associated protein (MAP), Tau primarily functions to protect microtubules (MTs) against depolymerization by decreasing the dissociation of tubulin at both MT ends, leading to an elevated growth rate and declined catastrophe frequency. In addition to being a stabilizer of microtubules, Tau is involved in multiple biological processes, including myelination, glucose metabolism, axonal transport, microtubule dynamics, iron homeostasis, neurogenesis, motor function, learning & memory, neuronal excitability, and DNA protection.

Sensitivity

3.9 pg/ml

Detection Method

Sandwich

Sample Type

serum, plasma, cerebrospinal fluid (CSF)

Assay Type

quantitative

Assay Time

3-5 working days

Assay Duration

1-5h

Precision

Intra-assay Precision (Precision within an assay): CV%<8%.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%.
Three samples of known concentration were tested in twenty assays to assess.

Number Of Samples

50-100ul

Detection Range

15.6 pg/ml-1000 pg/ml

Max Emission

450 nm

Target

Tau

Official Name

MAPT

Full Name

microtubule associated protein tau

Alternative Names

DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU, G protein beta1/gamma2 subunit-interacting factor 1, microtubule-associated protein tau, isoform 4

Gene ID

4137(Human)

Uniprot ID

P10636(Human)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.