NeuroMab™ Anti-CD4 BBB Shuttle Antibody(NRZP-1022-ZP3723)

Figure 1 shows the reactivity of anti-CD4 monoclonal antibodies against the human T-cell lymphoma cell line HPB-ALL in FCM analysis.

Figure 2 shows the complement-dependent cytotoxicity (CDC activity) of anti-CD4 rat monoclonal antibody and anti-CD4 mouse monoclonal antibody OKT4 on CD4 expressing transfectants.

The ordinate represents the cytotoxicity rate (%) of the cells, and the abscissa represents the antibody concentration of the anti-CD4 monoclonal antibody. ● denotes anti-CD4 rat monoclonal antibody KM4066, ▪ denotes anti-CD4 rat monoclonal antibody KM4067, - denotes anti-CD4 rat monoclonal antibody KM4068, and ◆ denotes anti-CD4 mouse monoclonal antibody OKT4.

Figure 3 shows the reactivity of various anti-CD4 antibodies in binding ELISA.

The horizontal axis represents the concentration of each antibody, and the vertical axis represents the binding activity of each antibody. The reactivity of each antibody against recombinant human CD4 is shown. Dotted line △ represents monoclonal antibody 6G5-1, solid line △ represents anti-CD4 human antibody 6G5-P, ▴ represents anti-CD4 human antibody 6G5-113F, ● represents anti-CD4 chimeric antibody KM4045, ▪ represents anti-CD4 chimeric antibody KM4046, □ represents anti-CD4 chimeric antibody KM4047, dotted line x represents anti-CD4 chimeric antibody KM4048, solid line x represents anti-CD4 chimeric antibody KM4049.

Figure 4 shows the antibody-dependent cellular cytotoxicity (ADCC activity) of various anti-CD4 antibodies against various human T cell lines.

The vertical axis represents the cytotoxicity rate (%) of the cells, and the horizontal axis represents the antibody concentration of each antibody. Dotted line Δ indicates monoclonal antibody 6G5-1, solid line Δ indicates monoclonal antibody 6G5-P, ▴ indicates chimeric antibody 6G5-113F, ● indicates chimeric antibody KM4045, ▪ indicates chimeric antibody KM4046, □ indicates chimeric antibody KM4047 , the dotted line x indicates the chimeric antibody KM4048, and the solid line x indicates the chimeric antibody KM4049. Fig. 9(A) shows the ADCC activity when HPB-ALL was used as the target cell. Fig. 9(B) shows the ADCC activity when HUT78 was used as the target cell. Fig. 9(C) shows ADCC activity when SUP-T1 was used as target cells. Fig. 9(D) shows the ADCC activity when HPB-ALL was used as the target cell.

Figure 5 shows the complement-dependent cytotoxicity (CDC activity) of various anti-CD4 antibodies against CD4-expressing transfectants or human lymphoma cell lines.

Figure 6 shows the reactivity of various anti-CD4 antibodies against the CD4-positive T-cell lymphoma cell line HPB-ALL using flow cytometry.

The abscissa represents the antibody concentration (μg/mL), and the ordinate represents the mean fluorescence intensity, MFI value. ● indicates the chimeric antibody KM4045, □ indicates the humanized antibody HV0LV0, ○ indicates the humanized antibody HV2LV0, ⋄ indicates the humanized antibody HV3LV0, - indicates the humanized antibody HV4LV0, x indicates the humanized antibody HV4LV6, the dotted line Δ represents human antibody 6G5-1, and Δ in a solid line represents human antibody 6G5-P.

Figure 7 shows the antibody-dependent cellular cytotoxicity (ADCC activity) of various anti-CD4 antibodies against various human T-cell lymphoma lines.

The vertical axis represents the cytotoxicity rate (%) of the cells, and the horizontal axis represents the antibody concentration (ng/mL). ● indicates chimeric antibody KM4045, ○ indicates humanized antibody KM8045, dotted line ▴ indicates human antibody 6G5-1, and solid line ▴ indicates human antibody 6G5-P. Fig. 12(A) shows the ADCC activity when HPB-ALL was used as the target cell. Fig. 12(B) shows the ADCC activity when HUT78 was used as the target cell. Fig. 12(C) shows the ADCC activity when CCRF-CEM was used as the target cell.

Figure 8 shows the antitumor effect of each antibody on the human T-cell lymphoma cell line HH transplanted into SCID mice (an early stage cancer model).

The abscissa indicates the days after tumor transplantation, and the ordinate indicates the tumor volume. x indicates negative control group, ▴ indicates KM8045 administered group, ● indicates 6G5-P administered group. Bars represent standard deviation.

Figure 9 shows the antitumor effect of individual antibodies on CD4/EL4 cells transplanted into C57BL/6 mice (metastasis model).

The ordinate represents the liver weight ratio. in Fig. In FIG. 14(B), the ordinate represents the kidney weight ratio. Bars represent standard deviation.

Figure 10 shows the antitumor effect of each antibody on the HH cell line transplanted into SCID mice (progressive cancer model).

The abscissa indicates the days after tumor transplantation, and the ordinate indicates the tumor volume. x represents the negative control group, ▴ represents the group administered with 20 μg/head of KM8045, ● represents the group administered with 100 μg/head of KM8045, and ▪ represents the group administered with 200 μg/head of KM8045 were administered. Bars represent standard deviation.