NeuroMab™ Anti-GARP BBB Shuttle Antibody(NRZP-1022-ZP3032)

Product Overview

Description

Brain uptake of therapeutic antibodies is severely limited by their size. To achieve enhanced BBB crossing, Creative Biolabs developed a BBB shuttle antibody platform by utilizing the endogenous macromolecule transportation pathway, known as receptor-mediated transcytosis (RMT). The engineered antibody-based carrier is believed to significantly to increase the macromolecule brain entry to combat CNS diseases.
Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.

Species Reactivity

Human; Cynomolgus Monkey

Clonality

Monoclonal

Host Species

Humanized

Applications

FC; Inhib; Block; In Vitro; In Vivo

Relevant Diseases

Amyotrophic Lateral Sclerosis

Product Properties

Storage

Store at -20°C. Do not aliquot the antibody.

Research Use Only

For research use only

Target

GARP

Official Name

LRRC32

Full Name

Leucine Rich Repeat Containing 32

Alternative Names

Leucine Rich Repeat Containing 32 ; Glycoprotein A Repetitions Predominant ; Transforming Growth Factor Beta Activator LRRC3; Leucine-Rich Repeat-Containing Protein 3; D11S833E ; Garpin ; GARP ; LRRC32

Product Pictures

FCM

Figure 1 Cross-reactivity with macaque GARP-TGFβ measured on cells by FACS. 293E cells were transfected with human/cynomolgus GARP and human/cynomolgus TGFB. LHG-10-D and affinity-optimized variants cross-react with cynomolgus GARP-TGFB.

Inhib

Figure 2 MHGARP8 and LHG-10 inhibit the suppressive activity of human Tregs in vitro.

Inhib

Figure 3 MHGARP8 and LHG-10 inhibit human Treg from producing active TGF-β.

After a short in vitro amplification, human CD4+CD25hiCD127lo cells (Tregs) were re-stimulated with anti-CD3/CD28 coated beads during 24 hours, in the presence or absence of the indicated mAbs (10 μg/ml). Cells lysates were analyzed by Western Blot with antibodies against phosphorylated SMAD2 (pSMAD2), as a read-out for active TGF-β production, or β-ACTIN (loading control).

Publications