NeuroMab™ Anti-PDGFRA Antibody(NRP-0422-P1969)

Product Overview

Description

The monoclonal antibody binds to human PDGFRa and neutralize activation of the receptor. The PDGFRa antagonist antibody is preferably one which inhibits binding of PDGFRa or neutralizes PDGFRa.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Human

Applications

WB; Inhib; Block; In Vitro; Antagonist; Neut

Relevant Diseases

Glioblastoma

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

> 95% (SDS-PAGE)

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Target

PDGFRA

Official Name

PDGFRA

Full Name

Platelet-derived Growth Factor Receptor alpha

Alternative Names

alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor; alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA

Gene ID

5156(Human); 18595(Mouse); 25267(Rat)

Uniprot ID

P16234(Human); P26618(Mouse); P20786(Rat)

Product Pictures

Figure 1 depicts the inhibition of Akt phosphorylation in PC3-ML cells by PDGFRα antagonists.

Panel A shows the dose dependent effect of monoclonal antibody IMC-3G3 on Akt phosphorylation induced by 30 ng/ml of PDGF-BB. Panels B provide a comparison of the effects of IMC-3G3 and AG1296 on bone marrow induced Akt phosphorylation.

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Figure 2 shows antibody binding to PDGFRα.

A: Direct binding of anti-PDGFRa antibody to the immobilized extracellular domain of PDGFRa

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Figure 3 shows antibody binding to PDGFRα.

B: Inhibition of [125I]PDGF-AA binding to immobilized PDGFRa.

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Figure 4 shows specific inhibition of phosphorylation of PDGFRα and downstream effector molecules.

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Figure 5 shows the inhibitory effect of mAbs on PDGF-AA-stimulated [3FI]thymidine incorporation in PAE Ra cells.

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Figure 6 shows inhibition of PDGF-AA-induced activation of downstream effector molecules in SKLMS-1 (A) and U118 (B) cells.

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Figure 7 shows that in vivo PDGFRα phosphorylation was reduced in U118 tumors treated with anti-PDGFRα antibody compared to treatment with non-specific human IgG.

Publications