NeuroMab™ Anti-CD195 BBB Shuttle Antibody(NRZP-1022-ZP3697)

Product Overview

Description

Brain uptake of therapeutic antibodies is severely limited by their size. To achieve enhanced BBB crossing, Creative Biolabs developed a BBB shuttle antibody platform by utilizing the endogenous macromolecule transportation pathway, known as receptor-mediated transcytosis (RMT). The engineered antibody-based carrier is believed to significantly to increase the macromolecule brain entry to combat CNS diseases.
Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Humanized

Isotype

IgG1

Applications

Block; Inhib; In Vivo; FC

Relevant Diseases

Multiple Sclerosis

Product Properties

Storage

Store at -20°C. Do not aliquot the antibody.

Research Use Only

For research use only

Target

CD195

Official Name

CCR5

Full Name

C-C Motif Chemokine Receptor 5

Alternative Names

C-C Motif Chemokine Receptor 5 (Gene/Pseudogene); Chemokine (C-C Motif) Receptor 5; HIV-1 Fusion Coreceptor; CC-CKR-5; ChemR13; CMKBR5; CCR-5; Chemokine (C-C Motif) Receptor 5 (Gene/Pseudogene); C-C Motif Chemokine Receptor 5 A159A; Chemokine Recptor CCR5 Delta32;

Product Pictures

FuncS

Figure 1 Sustained reduction in viral load.

SCID mice were reconstituted with normal human PBMCs and infected with HIV-1 JR-CSF. When viral steady state was reached, animals were treated ip every three days with a dose of 0.1 mg of a humanized CCR5 antibody (PRO140), and plasma HIV RNA (assayed by Roche Amplicor) was monitored.

FuncS

Figure 2 Humanized CCR5 antibody (PRO140) efficiently blocks CCR5-mediated HIV-1 cell fusion.

Mouse CCR5 antibody was humanized using complementarity determining region (CDR) grafting and framework replacement. Humanized CCR5 antibodies (PRO 140 #1 and PRO 140 #2) were expressed in Sp2/0 cells, purified by protein A chromatography and tested for their ability to block HIV-1JR-FL env-mediated replication of membrane fusion.

FuncS

Figure 3 Hu PRO140 blocks HIV-1 but not RANTES signaling.

The ability of PRO140 antibodies to block RANTES-induced calcium mobilization in L1.2-CCR5 cells was tested. This figure shows that a humanized CCR5 antibody (huPRO140) blocks HIV-1 but not RANTES signaling.

Publications