NeuroMab™ Anti-PDGFRA BBB Shuttle Antibody(NRZP-1022-ZP3337)

Product Overview

Description

Brain uptake of therapeutic antibodies is severely limited by their size. To achieve enhanced BBB crossing, Creative Biolabs developed a BBB shuttle antibody platform by utilizing the endogenous macromolecule transportation pathway, known as receptor-mediated transcytosis (RMT). The engineered antibody-based carrier is believed to significantly to increase the macromolecule brain entry to combat CNS diseases.
Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Human

Applications

WB; Inhib; Block; In Vitro; Antagonist; Neut

Relevant Diseases

Glioblastoma

Product Properties

Storage

Store at -20°C. Do not aliquot the antibody.

Research Use Only

For research use only

Target

PDGFRA

Official Name

PDGFRA

Full Name

Platelet-derived Growth Factor Receptor alpha

Alternative Names

alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor; alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA

Product Pictures

Figure 1 depicts the inhibition of Akt phosphorylation in PC3-ML cells by PDGFRα antagonists.

Panel A shows the dose dependent effect of monoclonal antibody IMC-3G3 on Akt phosphorylation induced by 30 ng/ml of PDGF-BB. Panels B provide a comparison of the effects of IMC-3G3 and AG1296 on bone marrow induced Akt phosphorylation.

FuncS

Figure 2 shows antibody binding to PDGFRα.

A: Direct binding of anti-PDGFRa antibody to the immobilized extracellular domain of PDGFRa

FuncS

Figure 3 shows antibody binding to PDGFRα.

B: Inhibition of [125I]PDGF-AA binding to immobilized PDGFRa.

FuncS

Figure 4 shows specific inhibition of phosphorylation of PDGFRα and downstream effector molecules.

FuncS

Figure 5 shows the inhibitory effect of mAbs on PDGF-AA-stimulated [3FI]thymidine incorporation in PAE Ra cells.

FuncS

Figure 6 shows inhibition of PDGF-AA-induced activation of downstream effector molecules in SKLMS-1 (A) and U118 (B) cells.

FuncS

Figure 7 shows that in vivo PDGFRα phosphorylation was reduced in U118 tumors treated with anti-PDGFRα antibody compared to treatment with non-specific human IgG.

Publications