NeuroMab™ Anti-SOD1 BBB Shuttle Antibody(NRZP-1022-ZP2579)

Fig.1 Western Dot Blot showing the binding of nanobodies to immobilized recombinant SOD1.

h3D6v2 was as effective as chimeric 3D6 in inducing phagocytosis of Aβ aggregates from PDAPP mouse brain tissue. IgG was used as a negative control in this experiment because it is unable to bind Aβ and therefore cannot induce phagocytosis.

Fig.2 SOD1 nanobodies reduce SOD1 aggregation in HeLa cells.

Expression of HA-tag Nb and SOD1-CFP in HeLa cells

Fig.3 The SOD1 nanobody reduces SOD activity.

When the SOD1 nanobody is added to the cell medium, the SOD activity is decreased. Read-outs are OD values, inversely correlated with SOD activity.

Fig.4 The survival and disease onset are increased in treated hSOD1G93A mice.

When the SOD1 nanobody is added to the cell medium, the SOD activity is decreased. Read-outs are OD values, inversely correlated with SOD activity.

Fig.5 The SOD1 nanobody rescues MN death.

The number of small MN, but not of big MN, was increased at P145 in mice treated with SOD1 nanobody from P60.

Fig.6 The SOD1 nanobody recognizes neurotoxic forms of SOD1.

The number of small MN, but not of big MN, was increased at P145 in mice treated with SOD1 nanobody from P60.