Human Superoxide dismutase [Cu-Zn] (SOD1) ELISA kit
[CAT#: NRZP-0722-ZP273]
For the quantitative determination of human superoxide dismutase [Cu-Zn] (SOD1) concentrations in serum, plasma, tissue homogenates.
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Product Overview
Description
This assay has high sensitivity and excellent specificity for detection of human SOD1.
Species Reactivity
Human
Marker
Superoxide dismutase 1, soluble
Properites
Sensitivity
In most cases, the sensitivity is below 3.9pg/ml.
Detection Method
Sandwich
Sample Type
serum, plasma, cell culture supernates
Assay Type
quantitative
Assay Duration
1-5h
Number Of Samples
50-100ul
Detection Range
15.6 pg/ml-1000 pg/ml
Max Emission
450 nm
Target Details
Protocols
Protocols
1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
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