Human Superoxide dismutase [Cu-Zn] (SOD1) ELISA kit

Name: Human Superoxide dismutase [Cu-Zn] (SOD1) ELISA kit
SKU: NRZP-0722-ZP273
Rating: 5 (1 reviews)

[CAT#: NRZP-0722-ZP273]

For the quantitative determination of human superoxide dismutase [Cu-Zn] (SOD1) concentrations in serum, plasma, tissue homogenates.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of human SOD1.

Species Reactivity

Human

Marker

Superoxide dismutase 1, soluble

Properites

Sensitivity

In most cases, the sensitivity is below 3.9pg/ml.

Detection Method

Sandwich

Sample Type

serum, plasma, cell culture supernates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

15.6 pg/ml-1000 pg/ml

Max Emission

450 nm

Target Details

Target

SOD1

Official Name

SOD1

Full Name

superoxide dismutase 1

Alternative Names

ALS; SOD; ALS1; IPOA; STAHP; hSod1; HEL-S-44; homodimer

Gene ID

6647(Human)

Uniprot ID

P00441(Human)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

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