NeuroMab™ Anti-ADAM17 BBB Shuttle Antibody(NRZP-1022-ZP3628)
- Host Species:
- Human
- Species Reactivity:
- Human
- Applications:
- Inhib; In Vitro
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
SPECIFIC INQUIRY
inquiryDescription
Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.
Species Reactivity
Clonality
Host Species
Applications
Relevant Diseases
Storage
Research Use Only
Target
Official Name
Full Name
Alternative Names
Figure 1. Results of experiments performed with the human breast cancer cell line MDA-MB-231.
Fig. A shows the results of experiments in which the effects of various anti-ADAM17 antibodies or antibody fragments on viability of MDA-MB-231 breast cancer cells were tested using an ALAMARBLUE cell viability assay. The effects of D5 IgG, D5 Fab, D8 IgG, and D8 Fab were tested. The control was an IgGl control. GM6001is a matrix metalloprotease inhibitor.
Figure 2. Experimental results using the human breast cancer cell line MDA-MB-231.
Fig. B-D present the results of experiments in which cultured MDA-MB-231 cells were stained with anti-ADAM17 antibodies D5 and D8 and alexa568-labeled anti-human IgG secondary antibody (green). As a control, cells were stained with the secondary antibody only. Nuclei were stained with Hoechst (blue) before imaging by confocal microscopy. Microscopic images of MDA-MB-231 cells stained for D5 (Figure 2B), D8 (Figure 2C), and control (Figure 2D) are shown, respectively.
Figure 3 shows the results of a dose-response experiment performed to assess the effect of the parental D8 clone and the three most potent affinity-matured clones derived from D8 on the proliferation of the MDA-MB-231 cell line - Quantitative Cell Viability Assay Using ALAMARBLUE .
Figure 4 shows the results of a dose-response experiment performed to assess the effect of two different batches of parental D8 clones (referred to as "D8 (NIH)" and "D8 (LP)" in the figure), and two Effect of the most potent affinity matured clones of D8 (ie, "D8.PI.Cl" and "D8.P2.C6.") on the proliferation of the MDA-MB-231 cell line - quantified using the ALAMARBLUE cell viability assay.
Figure 5. Proliferation inhibition of ovarian cancer cell line SKOV3 by anti-ADAM17 monoclonal antibody D8 and its affinity matured version D8P1C1.
Figure 6. Proliferation inhibition of ovarian cancer cell line CaoV3 by anti-ADAM17 monoclonal antibody D8 and its affinity matured version D8P1Cl.
Figure 7. Proliferation inhibition of the ovarian cancer cell line OVCAR-3 by the anti-ADAM17 monoclonal antibody D8 and its affinity-matured version, D8P1C1.
Figure 8. Proliferation inhibition of breast cancer cell line SKBR-3 by anti-ADAM17 monoclonal antibody D8 and its affinity matured version D8P1C1.
Figure 9. Proliferation inhibition of breast cancer cell line MCF-7 by anti-ADAM17 monoclonal antibody D8 and its affinity matured version D8P1C1.
Figure 10. Proliferation inhibition of colon cancer cell line LIM1215 by anti-ADAM17 monoclonal antibody D8 and its affinity matured version D8P1C1.
Figure 11. Proliferation inhibition of the glioblastoma cell line U87 MG by the anti-ADAM17 monoclonal antibody D8 and its affinity matured version D8P1C1.
Figure 13. Data showing the antitumor effect of mAh D8P1C1 in a triple negative breast cancer xenograft assay.
Inject 10 million MDA-MB-231 cells per mouse into 6-8 week old athymic nude mice (n=5). In the treatment group ("D8" in the figure), each mouse was injected with D8P1C1 (i.p.) at a dose of 15 mg/kg every two weeks for 4 weeks. In the control group, PBS was used as vehicle control ("Control" in the figure). The average tumor volume of the control and treatment groups was measured. The graph shows mean tumor volume (mm3) plotted against days post-injection. On the last day of the study, the percent (%) inhibition of tumor growth by D8P1C1 was 76.8% (calculated as 100 c [1 - [(TreatedFinal day - TreatedDay 1)/(ControlFinal day - ControlDay 1)]]).
Publications (0)
- NeuroMab™ Anti-Tau Antibody(NRP-0422-P1760) (Cat#: NRP-0422-P1760)
- NeuroMab™ Anti-ApoC3 BBB Shuttle Antibody(NRZP-1022-ZP3505) (Cat#: NRZP-1022-ZP3505)
- NeuroMab™ Anti-FGFR1 Antibody(NRP-0422-P1244) (Cat#: NRP-0422-P1244)
- NeuroMab™ Rabbit Anti-Alpha-synuclein (CBP1631) (Cat#: NAB-08-PZ079)
- NeuroMab™ Anti-SEZ6 Antibody(NRP-0422-P517) (Cat#: NRP-0422-P517)
- NeuroMab™ Anti-Alpha Synuclein Antibody(NRP-0422-P614) (Cat#: NRP-0422-P614)
- NeuroMab™ Anti-Tau Antibody(NRP-0422-P1686) (Cat#: NRP-0422-P1686)
- NeuroMab™ Mouse Anti-SHANK3 Monoclonal Antibody (CBP929) (Cat#: NAB-0720-Z3477)
- NeuroMab™ Anti-Integrin αvβ8 BBB Shuttle Antibody(NRZP-1222-ZP1218) (Cat#: NRZP-1222-ZP1218)
- NeuroMab™ Anti-SEZ6 Antibody(NRP-0422-P515) (Cat#: NRP-0422-P515)
- Rat Muller Cell (Cat#: NCL2110P040)
- Human Neurons Isolated from Cortex (Cat#: NCL-21P6-023)
- Rat Immortalized Retinal Muller Cell Line rMC-1 (Cat#: NCL-2106-S93)
- Human Astrocytes (Cat#: NCC20-9PZ01)
- Human Hippocampal Neuron Cells HPPNCs (Cat#: NCL2110P106)
- Rat Retinal Muller Cell Line, Immortalized (Cat#: NCL-21P6-192)
- iNeu™ Human Sensory Neurons (Cat#: NCL-2103-P62)
- Human Dental Pulp Stem Cells (Cat#: NRZP-1122-ZP113)
- Rat Schwann Cells RSC96, Immortalized (Cat#: NCL-2108P21)
- Mouse Glioma Cell Line GL261 (Cat#: NCL-2108P28)
- Alpha-Synuclein Aggregation Assay Kit (Cat#: NRZP-1122-ZP37)
- Human GFAP ELISA Kit [Colorimetric] (Cat#: NPP2011ZP383)
- Beta Amyloid (1-40), Aggregation Kit, TTF Assay (Cat#: NRZP-0323-ZP199)
- Human Poly ADP ribose polymerase,PARP Assay Kit (Cat#: NRZP-1122-ZP62)
- Beta Amyloid (1-42), Aggregation Kit (Cat#: NRZP-0323-ZP200)
- Amyloid beta 1-42 Kit (Cat#: NRP-0322-P2170)
- Alpha Synuclein Aggregation Kit (Cat#: NRZP-1122-ZP15)
- Human Tau Aggregation Kit (Cat#: NRP-0322-P2173)
- Dextran-FITC (Cat#: NTA-2011-ZP110)
- Dextran, NHS Activated, 40 kDa (Cat#: NRZP-0722-ZP124)
- VSV-eGFP (Cat#: NTA-2011-ZP20)
- pAAV-syn-FLEX-jGCaMP8f-WPRE (Cat#: NTA-2106-P064)
- rAAV-E-SARE-Cre-ERT2-PEST-WPRE-hGH polyA (Cat#: NTA-2010-TT342)
- pAAV-syn-FLEX-jGCaMP8m-WPRE (Cat#: NTA-2106-P065)
- pAAV-syn-jGCaMP8m-WPRE (Cat#: NTA-2106-P062)
- pAAV-syn-FLEX-jGCaMP8s-WPRE (Cat#: NTA-2106-P066)
- AAV2/2Retro-CAG-DIO-EGFP-2A-TetTox-pA [Neural Tracing] (Cat#: NTA-2012-ZP303)
- PRV-CAG-EGFP (Cat#: NTA-2011-ZP14)
- Rat Parkinson disease (autosomal recessive, juvenile) 2, parkin (Park2) (NM_020093) ORF clone/lentiviral particle, Myc-DDK Tagged (Cat#: NEP-0621-R0041)
- App Rat amyloid beta (A4) precursor protein (App)(NM_019288) ORF clone, Untagged (Cat#: NEP-0421-R0053)
- ABCA1 Antisense Oligonucleotide (NV-2106-P27) (Cat#: NV-2106-P27)
- Mouse Parkinson disease (autosomal recessive, early onset) 7 (Park7) (NM_020569) clone, Untagged (Cat#: NEP-0621-R0133)
- Human superoxide dismutase 1, soluble (SOD1) (NM_000454) ORF clone, TurboGFP Tagged (Cat#: NEP-0521-R0748)
- Mouse SOD1 shRNA Silencing Adenovirus (Cat#: NV-2106-P14)
- Tau Antisense Oligonucleotide (IONIS-MAPTRx) (Cat#: NV-2106-P29)
- Lenti of Human TAR DNA binding protein (TARDBP) (NM_007375) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0832)
- Human huntingtin-associated protein 1 (HAP1) transcript variant 2 (NM_177977) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0676)
- Lenti of Mouse synuclein, alpha (Snca) transcript variant (NM_001042451) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0864)
- NeuroBiologics™ Pig Cerebrospinal Fluid (Cat#: NRZP-0822-ZP498)
- NeuroBiologics™ Monkey Cerebrospinal Fluid (Cat#: NRZP-0822-ZP495)
- NeuroBiologics™ Rat Cerebrospinal Fluid (Cat#: NRZP-0822-ZP496)
- NeuroBiologics™ Mouse Cerebrospinal Fluid (Cat#: NRZP-0822-ZP497)
- NeuroBiologics™ Human Cerebrospinal Fluid (Cat#: NRZP-0822-ZP491)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein (Cat#: NRZP-0423-ZP498)
- NeuroPro™ Anti-NAGLU BBB Shuttle Protein (Cat#: NRZP-0423-ZP506)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein (Cat#: NRZP-0423-ZP501)
- NeuroPro™ Anti-SGSH BBB Shuttle Protein (Cat#: NRZP-0423-ZP505)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein (Cat#: NRZP-0423-ZP509)
- NeuroPro™ Anti-IDS BBB Shuttle Protein (Cat#: NRZP-0423-ZP503)
- NeuroPro™ Anti-idursulfase BBB Shuttle Protein (Cat#: NRZP-0423-ZP497)
- NeuroPro™ Anti-Erythropoietin BBB Shuttle Protein (Cat#: NRZP-0423-ZP499)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein (Cat#: NRZP-0423-ZP500)
- NeuroPro™ Anti-ASA BBB Shuttle Protein (Cat#: NRZP-0423-ZP504)