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NeuroMab™ Anti-Alpha Synuclein Antibody,Clone NR3463P

[CAT#: NRP-0422-P618]

A functional antibody raised against Human Alpha Synuclein.

Host Species:
Human
Species Reactivity:
Human
Applications:
WB; ELISA; IHC; In Vitro; In Vivo

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Product Overview

Description

NI-202.12F4 binds to α-synuclein fragments comprising aa 1-60 but not to N-terminally truncated fragments comprising amino acids 61-140 or 96-140. WB analysis shows that NI-202.12F4 selectively binds to α-synuclein. NI-202.12F4 selectively binds to very N-terminus of α-synuclein.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Human

Clone Number

NR3463P

Applications

WB; ELISA; IHC; In Vitro; In Vivo

Relevant Diseases

Parkinson's Disease
Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only
Target

Target

AlphaSynuclein

Official Name

SNCA

Full Name

Alpha-synuclein

Alternative Names

SNCA; NACP; PARK1; PARK4; PD1; synuclein alpha

Gene ID

6622 (Human); 20617 (Mouse)

Uniprot ID

Product Pictures
ELISA

Figure 1 Recombinant human α-synuclein antibodies against different epitopes.

Recombinant full-length and truncated α-synuclein were coated onto ELISA plates at the same coating concentration (20 μg/ml).

ELISA

Figure 2 Recombinant human α-synuclein antibody binds α-synuclein but not β- and γ-synuclein in a direct ELISA.

Recombinant α-, β- and γ-synuclein coated onto ELISA plates at the same coating concentration (2 μg/ml) together with recombinant human α-synuclein antibody or pan-synuclein antibody Incubation.

WB

Figure 3 Recombinant human α-synuclein antibody NI-202.12F4 binds α-synuclein but not β- and γ-synuclein in Western blot analysis.

Recombinant α-, β- and γ-synuclein (750 ng each) were subjected to SDS-PAGE followed by Western blot analysis.

ELISA

Figure 4. Recombinant NI-202.12F4 shows high affinity binding to wild-type and pathogenic α-synuclein mutants.

Recombinant wild type (●), A53T (▪), A30P (*) and E64K (⋄Human α-synuclein was coated on ELISA plates (2 μg/ml) and probed with different concentrations of NI202.12F4.

FuncS

Figure 5 Chronic treatment with NI-202.12F4 improved the locomotor capacity of α-synuclein A53T transgenic mice.

The time required to turn down was significantly reduced in NI-202.12F4-treated animals (t-turn; 1.7±0.3 vs. 4.6±0.6 s, p=0.0002, two-tailed Student's t-test).

FuncS

Figure 6 Chronic treatment of α-synuclein A53T transgenic mice with NI-202.12F4 results in increased plasma levels of human A53T α-synuclein.

Plasma samples were prepared from 12.5-month-old α-synuclein A53T transgenic mice treated with weekly intraperitoneal injections of 5 mg/kg NI-202.12F4 or PBS for 2 months. Blood was collected 24 hours after the last application.

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