CNS-related Cellular Kinase Assay Service
Our CNS-related cellular kinase assay service offers precise, high-throughput analysis of intracellular and receptor tyrosine kinases (RTKs). We deliver accurate measurements of kinase activity, compound binding, target protein affinity, and selectivity, empowering your research into complex central nervous system (CNS) signaling pathways and disease mechanisms. With exceptional sensitivity and specificity, our platform is ideal for studies ranging from basic research to drug discovery. For further information regarding the products and services provided, project-specific consultation, and pricing, please submit an inquiry here.
CNS-related Cellular Kinases
CNS-related kinases serve as pivotal regulators of neuronal development, synaptic plasticity, and energy metabolism, making them essential targets for elucidating the underlying mechanisms of neurological diseases. Understanding these kinases is fundamental to unraveling the complex pathologies of these disorders.
Fig.1 Kinase structure.1
Kinases | Description |
---|---|
CAMKII | CAMKII, a cornerstone of postsynaptic function, is critical for synaptic plasticity, learning, and memory. Its calcium-dependent activity drives long-term potentiation (LTP), making it a key player in neuronal signaling. |
CDK5 | CDK5, enriched in neurons, orchestrates cortical stratification, synaptic vesicle recycling, and cytoskeletal dynamics through its interaction with p35/p39. This kinase is essential for adult learning, memory, and synaptic function. |
CDKL5 | CDKL5 is vital during CNS development, regulating neuronal proliferation, migration, dendritic branching, and synaptic plasticity. Its cytoplasmic influence on the cytoskeleton directly impacts neural network formation. |
LKB1 kinase | LKB1 kinase governs axon development and neuronal polarity, and modulates neuronal metabolism and growth via downstream pathways like AMPK, highlighting its role in fundamental neuronal processes. |
SFKs | Src family kinases (SFKs), as non-receptor tyrosine kinases, modulate NMDA and P2X receptor signaling, thereby influencing synaptic transmission and plasticity. |
CKB | Highly expressed in the brain, creatine kinase (CKB) maintains neuronal energy homeostasis through the phosphocreatine-ATP cycle, buffering synaptic activity and ion pump function. |
MST kinases | MST kinases (MST1/2/3) participate in the MAPK signaling pathway, regulating cell proliferation, migration, and apoptosis, and are implicated in neurodevelopmental diseases and brain injury repair. |
AKAP12 | A-kinase anchoring protein 12 (AKAP12) acts as a scaffold, integrating PKA and PKC signals to influence blood-brain barrier development and long-term memory formation. |
CNS-related Cellular Kinase Assays
Traditional biochemical assays, limited by their simplified environments, often yield unreliable results due to the absence of crucial cellular complexities like membrane barriers, cofactor distribution, and pH gradients. Our advanced cell matrix analysis overcomes these limitations, providing accurate evaluations of key indicators:
- Target Affinity and Occupancy: Quantitatively measure real-time binding within cells, ensuring precise understanding of drug-target interactions.
- Cell Selectivity and Off-Target Effects: Screen against a comprehensive panel of 192-240 full-length kinases, revealing potential toxicity and ensuring target specificity.
- Direct Action Verification of Inhibitors: Distinguish whether compounds effectively penetrate cell membranes to engage their intended targets, validating true intracellular efficacy.
To receive customized testing solutions and limited-time discounts, please contact us today.
Reference
- Benn, Caroline L., and Lee A. Dawson. "Clinically precedented protein kinases: Rationale for their use in neurodegenerative disease." Frontiers in Aging Neuroscience 12 (2020): 242. Distributed under Open Access license CC BY 4.0, without modification.
- iNeuMab™ Anti-pTau Antibody (NRP-0422-P1719) (Cat#: NRP-0422-P1719)
- iNeuMab™ Anti-Tau Antibody (NRP-0422-P2293) (Cat#: NRP-0422-P2293)
- iNeuMab™ Anti-CD20 Antibody (NRP-0422-P1230) (Cat#: NRP-0422-P1230)
- iNeuMab™ Anti-Tau Antibody (NRP-0422-P1683) (Cat#: NRP-0422-P1683)
- iNeuMab™ Anti-ApoC3 BBB Shuttle Antibody (NRZP-1022-ZP3505) (Cat#: NRZP-1022-ZP3505)
- iNeuMab™ Anti-EPHB2 Antibody (NRP-0422-P1220) (Cat#: NRP-0422-P1220)
- iNeuMab™ Mouse Anti-LRP1 Monoclonal Antibody (CBP3363) (Cat#: NAB-0720-Z6479)
- iNeuMab™ Anti-SEZ6 Antibody (NRP-0422-P517) (Cat#: NRP-0422-P517)
- iNeuMab™ Anti-Amyloid Beta 1-15 Antibody (NRP-0422-P867) (Cat#: NRP-0422-P867)
- iNeuMab™ Anti-GD2 Antibody (NRZP-1222-ZP767) (Cat#: NRZP-1222-ZP767)
- Human Astrocytes, Immortalized (Cat#: NCL-2105-P182-AM)
- Human Blood Brain Barrier Model (Cat#: NCL-2103-P187)
- Mouse Glioma Cell Line GL261 (Cat#: NCL-2108P28)
- Human Brain Vascular Adventitial Fibroblasts (Cat#: NCL-21P6-014)
- Mouse Retinal Ganglion Cell Line RGC-5 (Cat#: NCL2110P154)
- Human Microglia Cell Line HMC3, Immortalized (Cat#: NCL-2108P38)
- Human Dental Pulp Stem Cells (Cat#: NRZP-1122-ZP113)
- Mouse Midbrain Dopaminergic Neuron Cell MN9D (Cat#: NCL2110P059)
- iNeu™ Human Motor Neurons (Cat#: NCL-2103-P71)
- Green Fluorescent Tau cell Line (Cat#: NCL2110P219)
- Beta Amyloid (1-42), Aggregation Kit (Cat#: NRZP-0323-ZP200)
- Beta Amyloid (1-40), Aggregation Kit (Cat#: NRZP-0323-ZP199)
- Amyloid beta 1-42 Kit (Cat#: NRP-0322-P2170)
- Human GFAP ELISA Kit [Colorimetric] (Cat#: NPP2011ZP383)
- Alpha Synuclein Aggregation Kit (Cat#: NRZP-1122-ZP15)
- Human Poly ADP ribose polymerase,PARP Assay Kit (Cat#: NRZP-1122-ZP62)
- Alpha-Synuclein Aggregation Assay Kit (Cat#: NRZP-1122-ZP37)
- Human Tau Aggregation Kit (Cat#: NRP-0322-P2173)
- AAV2 Full Capsids, Reference Standards (Cat#: NTC2101070CR)
- VSV-eGFP (Cat#: NTA-2011-ZP20)
- Dextran, NHS Activated (Cat#: NRZP-0722-ZP124)
- Human huntingtin (HTT) (NM_002111) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0497)
- ABCA1 Antisense Oligonucleotide (NV-2106-P27) (Cat#: NV-2106-P27)
- App Rat amyloid beta (A4) precursor protein (App)(NM_019288) ORF clone, Untagged (Cat#: NEP-0421-R0053)
- Lenti of Human TAR DNA binding protein (TARDBP) (NM_007375) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0832)
- Human huntingtin-associated protein 1 (HAP1) transcript variant 2 (NM_177977) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0676)
- Mouse Parkinson disease (autosomal recessive, early onset) 7 (Park7) (NM_020569) clone, Untagged (Cat#: NEP-0621-R0133)
- Human presenilin 1 (PSEN1), transcript variant 2 (NM_007318) ORF clone, TurboGFP Tagged (Cat#: NEP-0421-R0140)
- Tau Antisense Oligonucleotide (IONIS-MAPTRx) (Cat#: NV-2106-P29)
- Human superoxide dismutase 1, soluble (SOD1) (NM_000454) ORF clone, TurboGFP Tagged (Cat#: NEP-0521-R0748)
- Mouse SOD1 shRNA Silencing Adenovirus (Cat#: NV-2106-P14)
- NeuroBiologics™ Mouse Cerebrospinal Fluid (Cat#: NRZP-0822-ZP497)
- NeuroBiologics™ Monkey Cerebrospinal Fluid (Cat#: NRZP-0822-ZP495)
- NeuroBiologics™ Human Cerebrospinal Fluid (Cat#: NRZP-0822-ZP491)
- NeuroBiologics™ Pig Cerebrospinal Fluid (Cat#: NRZP-0822-ZP498)
- NeuroBiologics™ Rat Cerebrospinal Fluid (Cat#: NRZP-0822-ZP496)
- NeuroPro™ Anti-ASA BBB Shuttle Protein (Cat#: NRZP-0423-ZP504)
- NeuroPro™ Anti-IDS BBB Shuttle Protein (Cat#: NRZP-0423-ZP503)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein (Cat#: NRZP-0423-ZP500)
- NeuroPro™ Anti-SGSH BBB Shuttle Protein (Cat#: NRZP-0423-ZP505)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein (Cat#: NRZP-0423-ZP510)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein (Cat#: NRZP-0423-ZP509)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein (Cat#: NRZP-0423-ZP498)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein (Cat#: NRZP-0423-ZP502)
- NeuroPro™ Anti-PON1 BBB Shuttle Protein (Cat#: NRZP-0423-ZP507)
- NeuroPro™ Anti-Erythropoietin BBB Shuttle Protein (Cat#: NRZP-0423-ZP499)