CNS-related Cellular Kinase Assay Service

Our CNS-related cellular kinase assay service offers precise, high-throughput analysis of intracellular and receptor tyrosine kinases (RTKs). We deliver accurate measurements of kinase activity, compound binding, target protein affinity, and selectivity, empowering your research into complex central nervous system (CNS) signaling pathways and disease mechanisms. With exceptional sensitivity and specificity, our platform is ideal for studies ranging from basic research to drug discovery. For further information regarding the products and services provided, project-specific consultation, and pricing, please submit an inquiry here.

CNS-related Cellular Kinases

CNS-related kinases serve as pivotal regulators of neuronal development, synaptic plasticity, and energy metabolism, making them essential targets for elucidating the underlying mechanisms of neurological diseases. Understanding these kinases is fundamental to unraveling the complex pathologies of these disorders.

Fig. 1 Structure of Kinase. Fig.1 Kinase structure.¹

Kinases	Description
CAMKII	CAMKII, a cornerstone of postsynaptic function, is critical for synaptic plasticity, learning, and memory. Its calcium-dependent activity drives long-term potentiation (LTP), making it a key player in neuronal signaling.
CDK5	CDK5, enriched in neurons, orchestrates cortical stratification, synaptic vesicle recycling, and cytoskeletal dynamics through its interaction with p35/p39. This kinase is essential for adult learning, memory, and synaptic function.
CDKL5	CDKL5 is vital during CNS development, regulating neuronal proliferation, migration, dendritic branching, and synaptic plasticity. Its cytoplasmic influence on the cytoskeleton directly impacts neural network formation.
LKB1 kinase	LKB1 kinase governs axon development and neuronal polarity, and modulates neuronal metabolism and growth via downstream pathways like AMPK, highlighting its role in fundamental neuronal processes.
SFKs	Src family kinases (SFKs), as non-receptor tyrosine kinases, modulate NMDA and P2X receptor signaling, thereby influencing synaptic transmission and plasticity.
CKB	Highly expressed in the brain, creatine kinase (CKB) maintains neuronal energy homeostasis through the phosphocreatine-ATP cycle, buffering synaptic activity and ion pump function.
MST kinases	MST kinases (MST1/2/3) participate in the MAPK signaling pathway, regulating cell proliferation, migration, and apoptosis, and are implicated in neurodevelopmental diseases and brain injury repair.
AKAP12	A-kinase anchoring protein 12 (AKAP12) acts as a scaffold, integrating PKA and PKC signals to influence blood-brain barrier development and long-term memory formation.

CNS-related Cellular Kinase Assays

Traditional biochemical assays, limited by their simplified environments, often yield unreliable results due to the absence of crucial cellular complexities like membrane barriers, cofactor distribution, and pH gradients. Our advanced cell matrix analysis overcomes these limitations, providing accurate evaluations of key indicators:

Target Affinity and Occupancy: Quantitatively measure real-time binding within cells, ensuring precise understanding of drug-target interactions.
Cell Selectivity and Off-Target Effects: Screen against a comprehensive panel of 192-240 full-length kinases, revealing potential toxicity and ensuring target specificity.
Direct Action Verification of Inhibitors: Distinguish whether compounds effectively penetrate cell membranes to engage their intended targets, validating true intracellular efficacy.

To receive customized testing solutions and limited-time discounts, please contact us today.

Reference

Benn, Caroline L., and Lee A. Dawson. "Clinically precedented protein kinases: Rationale for their use in neurodegenerative disease." Frontiers in Aging Neuroscience 12 (2020): 242. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only. Not For Clinical Use.

In Vitro Services

Hot Products

Antibodies

Cells

Kits

Tracers

Gene Therapy Tools

Biospecimens

Cell Assay Reagents