Lipopolysaccharide (LPS)-induced Neuroinflammation Assay

Lipopolysaccharide (LPS)-induced neuroinflammation assay often employed in preclinical studies, holds immense significance in understanding the inflammatory response within the central nervous system. Creative Biolabs, with our vast experience in the field, offers a comprehensive LPS-induced Inflammation Assay that can revolutionize your research endeavors.

The Role of Lipopolysaccharide (LPS)

• LPS Structure

Lipopolysaccharide is a complex molecule composed of lipid A, core oligosaccharides, and O-antigen. It is known for its ability to activate the immune system, particularly through Toll-like receptor 4 (TLR4). This interaction between LPS and TLR4 initiates a cascade of pro-inflammatory responses, making LPS a potent tool for studying inflammation.

• Significance in Neuroinflammation

In the field of neuroscience, neuroinflammation has emerged as a central focus. Neuroinflammatory processes are implicated in various neurological disorders, including Alzheimer's disease, Parkinson's disease, and multiple sclerosis. Understanding the mechanisms behind LPS-induced inflammation is paramount for deciphering its role in neurological diseases.

Fig.1 Summarized downstream effects of LPS signaling. (Page, 2022)

LPS-induced Inflammation Assay

The LPS-induced inflammation assay involves exposing cells or organisms to LPS and monitoring the subsequent immune response. Key aspects of this assay include:

• Cell Culture Models: In vitro cell culture models using microglia, astrocytes, or neurons are commonly employed to investigate the effects of LPS.
• In Vivo Models: Animal models, such as mice or rats, can also be used to assess systemic and local inflammation induced by LPS.
• Measurement of Inflammatory Markers: Cytokines, chemokines, and inflammatory mediators like IL-1β, TNF-α, and NF-κB are often measured to assess the inflammatory response.

Why Choose Us?

• Precision in Experimental Design

At Creative Biolabs, we understand the importance of precision in experimental design. Our LPS-induced Inflammation Assay is tailored to meet the specific needs of your research. We offer a range of LPS concentrations, treatment durations, and cell types to ensure that the assay closely mimics the conditions of your study. This flexibility is essential for the accurate modeling of the inflammatory response within the central nervous system.

• State-of-the-Art Techniques

Our team of experts employs state-of-the-art techniques in conducting LPS-induced Inflammation Assays. We utilize primary neural cell cultures, microglia, and astrocytes to create a physiologically relevant in vitro model. By stimulating these cells with LPS, we can assess the production of pro-inflammatory cytokines and other inflammatory markers.

• Multiparametric Analysis

To provide a comprehensive assessment of the inflammatory response, we employ multiparametric analysis. Our assay evaluates a spectrum of parameters, including the release of cytokines, cell viability, and gene expression profiles. This multifaceted approach ensures that no aspect of the inflammatory process is overlooked.

If you are interested in our LPS-induced Inflammation Assay, please feel free to contact us for more information.

Reference

1. Page, M.J.; et al. The Role of Lipopolysaccharide-Induced Cell Signaling in Chronic Inflammation. Chronic Stress. 2022, 6: 24705470221076390.