Lipopolysaccharide (LPS) induced Neuroinflammation Assay Service

Neuroinflammation is a central pathological mechanism in neurodegenerative diseases such as Alzheimer's disease (AD), Parkinson's disease (PD), and multiple sclerosis (MS). Lipopolysaccharide (LPS), a component of the outer membrane of Gram-negative bacteria, can accurately simulate the molecular and behavioral characteristics of neuroinflammation by activating microglia, inducing the release of proinflammatory factors (such as TNF-α and IL-1β), and triggering the TLR4/NF-κB signaling pathway. This service, based on the internationally leading LPS-induced model, provides an efficient and reliable experimental platform for drug screening and mechanism research. For further information regarding the products and services provided, project-specific consultation, and pricing, please submit an inquiry here.

Applications of the LPS-Induced Neuroinflammation Model

The LPS-induced neuroinflammation model is a critical tool in a variety of research domains:

• Neurodegenerative Disease Studies

This model enables researchers to examine the contributions of LPS to the pathogenic processes of neurodegenerative disorders, including Alzheimer's and Parkinson's diseases. This allows for a deeper understanding of inflammatory pathways involved in disease progression.

• Anti-inflammatory Drug Evaluation

The LPS model serves as a platform for rigorous screening of potential anti-inflammatory therapeutic agents. Researchers can assess the efficacy of these compounds in mitigating neuroinflammatory responses.

• Molecular Mechanism Investigations

Scientists utilize this model to elucidate the precise molecular pathways activated by LPS, such as the Toll-like receptor 4 (TLR4) signaling cascade and the induction of oxidative stress. This research provides fundamental insights into the inflammatory process.

Advantages of Our Services

We deliver superior in vitro LPS-induced neuroinflammation testing, characterized by:

• Exceptional Accuracy and Precision

Leveraging state-of-the-art detection methodologies, including Enzyme-Linked Immunosorbent Assay (ELISA), quantitative Polymerase Chain Reaction (qPCR), and flow cytometry, we guarantee highly sensitive and specific results.

• Customizable Experimental Protocols

Our flexible service allows for tailored experimental designs, accommodating diverse research needs with variable LPS dosages, time-course analyses, and a wide array of cell types.

• In-Depth Data Interpretation

We provide comprehensive data analysis and detailed interpretive reports, empowering researchers with profound insights into the molecular mechanisms of LPS-induced neuroinflammation.

• Responsive Technical Support

Our dedicated team of scientific experts ensures prompt and efficient service, delivering a seamless research experience.

Case Study: LPS-induced pro-inflammatory cytokine production can be attenuated by mTOR inhibition

Fig.1 Dot-blotting analysis of four groups (Control, Rapamycin, LPS and LPS + Rapamycin). Each sample was analyzed in duplicates, and representative images for each group are shown here.

Fig.2 Relative changes of relevant pro-inflammatory cytokines after treatments with LPS, Rapamycin and LPS + Rapamycin. lmage quantifications were carried out using a customized software pipeline and graphs were generated with GraphPad Prism.

To discuss your experimental plan and receive a quote, please contact us today.