Neurogenesis Assay

A comprehensive study of neurogenesis allows scientists to better study numerous neurodegenerative disorders and for this purpose develop new therapies. As an expert in the field of neuroscience, Creative Biolabs is committed to providing innovative and professional neurogenesis assays to customers around the world.

Background of Neurogenesis

The central nervous system (CNS) is one of the most complex and important organs in mammals. Studying the development, function, and pathology of the CNS can help researchers obtain a more comprehensive understanding of the neurological mechanisms of action, the pathological mechanisms of neurological diseases, and the development of related drugs.

The nervous system is composed of a large number of nerve cells such as astrocytes and oligodendrocytes, as well as non-neural cells such as microglia, endothelial cells, fibroblasts, and blood cells, allowing for a highly complex wiring system with the ability to receive, integrate, and respond to a wide range of stimuli in a prompt and organized manner, and to maintain proper functions of neuronal cells in a balanced microenvironment. Dysfunction, loss, or impairment of neuronal cells are intimately related to multiple neurological disorders. Neuronal plasticity is characterized by the capacity of the nervous system to modify its structure and function in response to external environmental changes. Neural stem cells are capable of producing novel neural cells of any type, the process of which produced new neurons and nervous system cells is neurogenesis. Neurogenesis plays an essential role in neuronal plasticity, which is critical in brain development and maintaining the balance between neuronal cells and dead cells in the brain.

Our Comprehensive Neurogenesis Assay Services

Creative Biolabs develops therefore neurogenesis assays based on in vitro cell culture models for researchers to detect neurogenesis impairment or how the target compounds affect the neurogenesis.

• Induced pluripotent stem cells (iPSCs) as an in vitro neurogenesis model to imitate the early stages of nervous system development that can be used to study the generation of different subtypes of neuronal cells.
• The capture of an intermediate stage of neural progenitor cells (NPCs) in a controlled medium with growth factors can be used to differentiate stem cells.
• NPCs niche can be utilized to study the differentiation, proliferation, interaction, microenvironment, and neurogenesis among the resident cells.
• Neurosphere assay and neuroblast assay are also expedient in vitro assays that can be used to recur neurogenesis, especially to study the differentiation capabilities and to screen neurogenic or gliogenic compounds.
• Immunofluorescence analysis is applied for neural cell cultures.
• Digital images is used to compare the neurons.

Fig.1 The NeuroBlast assay (NBA) culture as an in vitro neurogenesis model. (Azari & Reynolds, 2016)

Creative Biolabs offers our clients customized in vitro neurogenesis assays based on different cell culture models, which enable researchers to comprehensively understand the mechanisms of neurogenesis and therefore discover and develop promising therapeutics. For further information, please don’t hesitate to contact us.

Reference

1. Azari, H.; Reynolds, B. A. In vitro models for neurogenesis. Cold Spring Harbor perspectives in biology. 2016, 8(6): a021279.