Zebrafish High-Content Phenotypic Screening Assay
Creative Biolabs is a leading company in the field of biomedical research and development, with a strong focus on innovative solutions in neuroscience. With over 20 years of experience in the industry, our team of expert biological specialists combines their extensive knowledge with cutting-edge technologies to provide our customers with exceptional Zebrafish-based Phenotyping/Screening/Imaging Service.
Zebrafish Phenotyping/Screening Assays
At Creative Biolabs, we offer a wide range of zebrafish phenotyping assays to help researchers better understand the biological mechanisms underlying various diseases. Our phenotyping assays include behavioral assays, physiological assays, and morphological assays. By employing zebrafish as an in vivo model, our approach enables target-independent and results-oriented drug discovery right from the outset. This methodology places emphasis on improving phenotypes, enabling the identification of effective drugs in a faster and more streamlined manner, ultimately saving valuable time and resources. Moreover, with recent advancements in genetics allowing for the reliable expression of human proteins, the translational potential of the zebrafish model has reached unprecedented levels. Our services cater to various project needs, including:
- Exploring alternative therapeutic indications (indication expansion) for your compounds.
- Discovering new therapeutic applications for existing drug targets.
- Identifying novel therapeutics to aid in the treatment of diseases.
- Validating drug targets identified in GWAS (Genome-Wide Association Studies) studies.
Fig.1 Using Zebrafish for small molecules drugs based high-throughput screening. (Kithcart,
2017)
Zebrafish High-Throughput Imaging Assays
In addition to traditional phenotyping assays, we also offer zebrafish high-throughput imaging assays to accelerate the drug discovery process. Our high-throughput imaging assays involve using automated imaging systems to capture images of large numbers of zebrafish in a short period.
Using this approach, we can quickly screen large libraries of potential therapeutic compounds and identify those with the most promising biological effects. Our team can also use imaging techniques to study the cellular and molecular mechanisms underlying various diseases and drug treatments.
Our Solutions
We offer comprehensive end-to-end solutions that encompass various stages of the neuroresearch process. Our solutions include:
Target Screening
- Disease Model Creation (Genetically modified/Knockout/Knock-in/Point mutation/ Humanized)
- Library Screening
- Disease Relevant Endpoints Analysis (Phenotypic/Molecular/Protein Analysis/Imaging)
Target Validation
- Crispants/Disrupt Genes
- Screen Disease Relevant Endpoints (Phenotypic/Molecular/Protein Analysis/Imaging/Gene Expression)
- Identify & Confirm Hits
Neurotoxicity Screening
- Embryo Acute Toxicity Testing
- Molecular Testing (Perform gene expression studies to characterize mechanisms of action)
- Other Toxicity Testing (Hepatotoxicity Testing/ Cardiotoxicity Testing)
Therapeutic Testing
- Disease Model Creation (Genetically modified/Knockout/Knock-in/Point mutation/ Humanized)
- Therapeutic Testing (Small Molecule/ ASO / mRNA /Biologics)
- Disease Relevant Endpoints Analysis (Phenotypic/Molecular/Protein Analysis/Imaging)
Our General Workflow
Project Design
We will define the scope of your project and create a proposal that includes cost and time estimate for each experiment proposed. You will be able to personalize your project plan.
Project Finalization
Upon receipt of your first purchase order (PO), we will commence the experimentation phase and provide you with a detailed project timeline. Additionally, we will furnish you with a final statement of work and payment schedule.
Project Report Out
We are committed to providing you with regular updates on the status of your project, with a frequency of once every two weeks. Furthermore, upon the completion of your project, we will furnish you with a comprehensive report.
Please feel free to contact us to learn more about our Zebrafish Phenotyping and Screening Services and how we can accelerate your drug discovery process.
Reference
- Kithcart, Aaron, and Calum A. MacRae. "Using zebrafish for high-throughput screening of novel cardiovascular drugs." Basic to Translational Science 2.1 (2017): 1-12.
