Zebrafish High-Content Phenotypic Screening Assay Service
Creative Biolabs is a leading company in the field of biomedical research and development, with a strong focus on innovative solutions in neuroscience. With over 20 years of experience in the industry, our team of expert biological specialists combines their extensive knowledge with cutting-edge technologies to provide our customers with exceptional Zebrafish-based Phenotyping/Screening/Imaging Service.
Zebrafish Phenotyping/Screening Assays
At Creative Biolabs, we offer a wide range of zebrafish phenotyping assays to help researchers better understand the biological mechanisms underlying various diseases. Our phenotyping assays include behavioral assays, physiological assays, and morphological assays. By employing zebrafish as an in vivo model, our approach enables target-independent and results-oriented drug discovery right from the outset. This methodology places emphasis on improving phenotypes, enabling the identification of effective drugs in a faster and more streamlined manner, ultimately saving valuable time and resources. Moreover, with recent advancements in genetics allowing for the reliable expression of human proteins, the translational potential of the zebrafish model has reached unprecedented levels. Our services cater to various project needs, including:
- Exploring alternative therapeutic indications (indication expansion) for your compounds.
- Discovering new therapeutic applications for existing drug targets.
- Identifying novel therapeutics to aid in the treatment of diseases.
- Validating drug targets identified in GWAS (Genome-Wide Association Studies) studies.
Fig.1 Using Zebrafish for small molecules drugs based high-throughput screening. (Kithcart,
2017)
Zebrafish High-Throughput Imaging Assays
In addition to traditional phenotyping assays, we also offer zebrafish high-throughput imaging assays to accelerate the drug discovery process. Our high-throughput imaging assays involve using automated imaging systems to capture images of large numbers of zebrafish in a short period.
Using this approach, we can quickly screen large libraries of potential therapeutic compounds and identify those with the most promising biological effects. Our team can also use imaging techniques to study the cellular and molecular mechanisms underlying various diseases and drug treatments.
Our Solutions
We offer comprehensive end-to-end solutions that encompass various stages of the neuroresearch process. Our solutions include:
Target Screening
- Disease Model Creation (Genetically modified/Knockout/Knock-in/Point mutation/ Humanized)
- Library Screening
- Disease Relevant Endpoints Analysis (Phenotypic/Molecular/Protein Analysis/Imaging)
Target Validation
- Crispants/Disrupt Genes
- Screen Disease Relevant Endpoints (Phenotypic/Molecular/Protein Analysis/Imaging/Gene Expression)
- Identify & Confirm Hits
Neurotoxicity Screening
- Embryo Acute Toxicity Testing
- Molecular Testing (Perform gene expression studies to characterize mechanisms of action)
- Other Toxicity Testing (Hepatotoxicity Testing/ Cardiotoxicity Testing)
Therapeutic Testing
- Disease Model Creation (Genetically modified/Knockout/Knock-in/Point mutation/ Humanized)
- Therapeutic Testing (Small Molecule/ ASO / mRNA /Biologics)
- Disease Relevant Endpoints Analysis (Phenotypic/Molecular/Protein Analysis/Imaging)
Our General Workflow
Project Design
We will define the scope of your project and create a proposal that includes cost and time estimate for each experiment proposed. You will be able to personalize your project plan.
Project Finalization
Upon receipt of your first purchase order (PO), we will commence the experimentation phase and provide you with a detailed project timeline. Additionally, we will furnish you with a final statement of work and payment schedule.
Project Report Out
We are committed to providing you with regular updates on the status of your project, with a frequency of once every two weeks. Furthermore, upon the completion of your project, we will furnish you with a comprehensive report.
Please feel free to contact us to learn more about our Zebrafish Phenotyping and Screening Services and how we can accelerate your drug discovery process.
Reference
- Kithcart, Aaron, and Calum A. MacRae. "Using zebrafish for high-throughput screening of novel cardiovascular drugs." Basic to Translational Science 2.1 (2017): 1-12.
- iNeuMab™ Anti-Tau Antibody (NRP-0422-P2293) (Cat#: NRP-0422-P2293)
- iNeuMab™ Mouse Anti-EFNB2 Monoclonal Antibody (CBP1159) (Cat#: NAB-0720-Z4396)
- iNeuMab™ Anti-Tau Antibody (NRP-0422-P1684) (Cat#: NRP-0422-P1684)
- iNeuMab™ Anti-SEZ6 Antibody (NRP-0422-P517) (Cat#: NRP-0422-P517)
- iNeuMab™ Anti-Integrin αvβ8 BBB Shuttle Antibody (NRZP-1222-ZP1218) (Cat#: NRZP-1222-ZP1218)
- iNeuMab™ Anti-TREM2 Antibody (NRP-0422-P792) (Cat#: NRP-0422-P792)
- iNeuMab™ Anti-Tau Antibody (NRP-0422-P1683) (Cat#: NRP-0422-P1683)
- iNeuMab™ Rabbit Anti-Alpha-synuclein (CBP1631) (Cat#: NAB-08-PZ079)
- iNeuMab™ Mouse Anti-LRP1 Monoclonal Antibody (CBP3363) (Cat#: NAB-0720-Z6479)
- Mouse Anti-SCN5A Monoclonal Antibody (CBP708) (Cat#: NAB-0720-Z2720)
- Rat Glioma Cell Line C6 (Cat#: NCL2110P346)
- Mouse Microglia N9 (Cat#: NCL2110P073)
- Human Astrocytes, Immortalized (Cat#: NCL-2105-P182-AM)
- Rat Olfactory Ensheathing Cells (Cat#: NRZP-1122-ZP162)
- Human Brain Astroblastoma U-87 MG (Cat#: NCL2110P117)
- Human Brain Microvascular Endothelial Cells (Cat#: NCL-2103-P133)
- iNeu™ Human Oligodendrocyte Progenitor Cells (OPCs) (Cat#: NCL-2103-P49)
- Green Fluorescent Tau cell Line (Cat#: NCL2110P219)
- Mouse Microglia from C57BL/6 (Cat#: NCL-21P6-082)
- Mouse Midbrain Dopaminergic Neuron Cell MN9D (Cat#: NCL2110P059)
- Beta Amyloid (1-42), Aggregation Kit (Cat#: NRZP-0323-ZP200)
- Beta Amyloid (1-40), Aggregation Kit (Cat#: NRZP-0323-ZP199)
- Human Tau Aggregation Kit (Cat#: NRP-0322-P2173)
- Amyloid beta 1-42 Kit (Cat#: NRP-0322-P2170)
- Human Poly ADP ribose polymerase,PARP Assay Kit (Cat#: NRZP-1122-ZP62)
- Human GFAP ELISA Kit [Colorimetric] (Cat#: NPP2011ZP383)
- Alpha Synuclein Aggregation Kit (Cat#: NRZP-1122-ZP15)
- Alpha-Synuclein Aggregation Assay Kit (Cat#: NRZP-1122-ZP37)
- Dextran, NHS Activated (Cat#: NRZP-0722-ZP124)
- AAV2/9-hEF1a-fDIO-eNpHR 3.0-mCherry-WPRE-pA (Cat#: NTA-2012-ZP78)
- AAV2 Full Capsids, Reference Standards (Cat#: NTC2101070CR)
- VSV-eGFP (Cat#: NTA-2011-ZP20)
- Rat Parkinson disease (autosomal recessive, juvenile) 2, parkin (Park2) (NM_020093) ORF clone/lentiviral particle, Myc-DDK Tagged (Cat#: NEP-0621-R0041)
- Human apolipoprotein E (APOE) (NM_000041) ORF clone, Untagged (Cat#: NEP-0421-R0232)
- Human huntingtin (HTT) (NM_002111) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0497)
- Lenti of Human TAR DNA binding protein (TARDBP) (NM_007375) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0832)
- Human superoxide dismutase 1, soluble (SOD1) (NM_000454) ORF clone, TurboGFP Tagged (Cat#: NEP-0521-R0748)
- Human superoxide dismutase 3, extracellular (SOD3) (NM_003102) ORF clone, Untagged (Cat#: NEP-0521-R0808)
- App Rat amyloid beta (A4) precursor protein (App)(NM_019288) ORF clone, Untagged (Cat#: NEP-0421-R0053)
- Mouse Parkinson disease (autosomal recessive, early onset) 7 (Park7) (NM_020569) clone, Untagged (Cat#: NEP-0621-R0133)
- Human presenilin 1 (PSEN1), transcript variant 2 (NM_007318) ORF clone, TurboGFP Tagged (Cat#: NEP-0421-R0140)
- Human huntingtin-associated protein 1 (HAP1) transcript variant 2 (NM_177977) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0676)
- NeuroBiologics™ Human Cerebrospinal Fluid (Cat#: NRZP-0822-ZP491)
- NeuroBiologics™ Rat Cerebrospinal Fluid (Cat#: NRZP-0822-ZP496)
- NeuroBiologics™ Mouse Cerebrospinal Fluid (Cat#: NRZP-0822-ZP497)
- NeuroBiologics™ Monkey Cerebrospinal Fluid (Cat#: NRZP-0822-ZP495)
- NeuroBiologics™ Pig Cerebrospinal Fluid (Cat#: NRZP-0822-ZP498)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein (Cat#: NRZP-0423-ZP509)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein (Cat#: NRZP-0423-ZP500)
- NeuroPro™ Anti-idursulfase BBB Shuttle Protein (Cat#: NRZP-0423-ZP497)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein (Cat#: NRZP-0423-ZP510)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein (Cat#: NRZP-0423-ZP498)
- NeuroPro™ Anti-EPO BBB Shuttle Protein (Cat#: NRZP-0423-ZP508)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein (Cat#: NRZP-0423-ZP502)
- NeuroPro™ Anti-NAGLU BBB Shuttle Protein (Cat#: NRZP-0423-ZP506)
- NeuroPro™ Anti-ASA BBB Shuttle Protein (Cat#: NRZP-0423-ZP504)
- NeuroPro™ Anti-PON1 BBB Shuttle Protein (Cat#: NRZP-0423-ZP507)