Service

C. elegans Overexpression Model Development Service

At Creative Biolabs, we are at the forefront of innovative research and development in the field of neuroscience. With over 20 years of experience, we specialize in providing cutting-edge services for the development of C. elegans overexpression models for studying neurological diseases. Our expertise lies in utilizing the powerful genetic toolkit of C. elegans to engineer transgenic strains that overexpress specific genes of interest.

C. elegans Overexpression Models for Neurological Diseases

By introducing and overexpressing disease-related genes in C. elegans, we can artificially increase the expression levels of specific genes associated with neurological disorders. This enables researchers to study the effects of gene alterations on neuronal function and behavior. We can selectively upregulating target genes associated with diseases such as:

Alzheimer's
Parkinson's
Huntington's
Dementia
Depression
Epilepsy

Motor disorder
Pain
Anxiety
Neuropathic
Neurodegeneration

Workflow for Generating Overexpressed C. elegans Models

Our C. elegans overexpression model development service follows a meticulously designed workflow to ensure the generation of reliable and reproducible models.

Gene Cloning and Transgenesis

Advanced molecular techniques are used to generate expression constructs. These constructs are then introduced into C. elegans using transgenic methods such as microinjection or biolistic bombardment.

Generation of Transgenic Strains

We carefully screen and select the transgenic worms that have successfully incorporated the overexpression construct into their genome. These strains become the basis for establishing stable lines that exhibit sustained and heritable overexpression of the target gene.

Screening and Validation

The injected worms are subjected to screening procedures to identify transgenic individuals that carry the desired overexpression construct. We utilize various molecular (WB, qPCR) and imaging techniques to confirm. the successful generation of transgenic lines.

Phenotypic Characterization

We conduct comprehensive phenotypic characterization of the generated C. elegans overexpression models, including assessing behavioral changes, neuronal morphology, synaptic functions, and other relevant physiological parameters.

Available C. elegans Models

Creative Biolabs offers a wide range of C. elegans overexpression models for studying various neurological diseases. Our team of experts collaborates closely with researchers to identify the target genes of interest, taking into account their relevance to specific neurological diseases. These models encompass different genes associated with specific disorders and can be tailored to meet the specific needs of researchers. Some of our available C. elegans overexpression models include:

Alzheimer's Disease:

BIN1 (Bridging integrator 1)	APP (Amyloid precursor protein)
CD33 (Cluster of Differentiation 33)	APOE (Apolipoprotein E)
PSENEN (Presenilin enhancer gamma-secretase subunit)	TREM2 (Triggering receptor expressed on myeloid cells 2)
ABCA7 (ATP-binding cassette subfamily A member 7)	CLU (Clusterin)
PTK2B (Protein tyrosine kinase 2 beta)	PICALM (Phosphatidylinositol binding clathrin assembly protein)

Parkinson's Disease:

SNCAIP (Synuclein alpha interacting protein)	GBA (Glucocerebrosidase)
ATP13A2 (ATPase cation transporting 13A2)	PARK2 (Parkin)
FBXO7 (F-box protein 7)	PARK7 (DJ-1)
GIGYF2 (GRB10 interacting GYF protein 2)	LRRK2 (Leucine-rich repeat kinase 2)
PARK16 (Parkinson disease 16)	VPS35 (Vacuolar protein sorting-associated protein 35)

Amyotrophic Lateral Sclerosis (ALS):

ANG (Angiogenin)	SOD1 (Superoxide dismutase 1)
OPTN (Optineurin)	C9orf72 (Chromosome 9 open reading frame 72)
NEK1 (NIMA-related kinase 1)	TARDBP (TAR DNA-binding protein 43)
FGGY (FGGY carbohydrate kinase domain containing)	FUS (Fused in sarcoma)
VCP (Valosin-containing protein)	UBQLN2 (Ubiquilin 2)

Huntington's Disease:

DNAJC14 (DnaJ heat shock protein family member C14)	HTT (Huntingtin)
PDE10A (Phosphodiesterase 10A)	PGC-1α (PPARG coactivator 1 alpha)
HIP1 (Huntingtin-interacting protein 1)	MSH3 (MutS homolog 3)
RANBP17 (RAN binding protein 17)	BDNF (Brain-derived neurotrophic factor)
PDE3A (Phosphodiesterase 3A)	Rhes (Ras homolog enriched in the striatum)

Epilepsy:

GABRG2 (Gamma-aminobutyric acid receptor gamma-2 subunit)	SCN1A (Sodium channel, voltage-gated, type I alpha subunit)
KCNT1 (Potassium channel subfamily T member 1)	KCNQ2 (Potassium voltage-gated channel subfamily Q member 2)
SLC2A1 (Solute carrier family 2 member 1)	GABRA1 (Gamma-aminobutyric acid receptor alpha-1 subunit)
LGI1 (Leucine-rich glioma-inactivated 1)	PRRT2 (Proline-rich transmembrane protein 2)
SLC25A22 (Solute carrier family 25 member 22)	GRIN2A (Glutamate receptor, ionotropic NMDA 2A)

Our repository of C. elegans overexpression models continue to expand as we collaborate with leading research institutions and actively contribute to the advancement of neuroscience. Moreover, we offer customizable services, allowing clients to tailor the development of overexpression models to their specific research needs. Please feel free to contact us for more about our C. elegans models-related services.

For Research Use Only. Not For Clinical Use.