Synaptogenesis Assay

In order to understand the interaction between synaptic activity and neurophysiology and pathology, explorations are required to investigate the molecular mechanisms involved in synaptogenesis, synaptic regulation, and synaptic plasticity. Creative Biolabs offers a range of synaptogenesis assays that allow for extensive studies of the microstructure of synaptic junctions based on immunofluorescence detection to identify mechanisms involved in synaptogenesis and neuronal plasticity as well as to drive drug screening.

What is Synaptogenesis?

Synapses are specialized interconnected neuronal junctions. The molecular network between synapses controls synaptic signaling transmission and neuronal plasticity that modulates neuronal growth, differentiation, and apoptosis. Action potentials in presynaptic axons stimulate the release of chemical neurotransmitters that activate specific receptor proteins in the postsynaptic and presynaptic membranes to transmit signals.

Synaptogenesis is a comprehensive process including synapse formation and function maintenance, which exerts an essential role in the brain development besides neurogenesis, migration, and pathfinding. Defective synaptogenesis usually results in the pathology of multiple neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease, Huntington's disease, Niemann Pick disease, etc. Many neurodegenerative disorders might benefit from a treatment strategy for enhancing synaptogenesis.

Fig.1 Temporal sequence of synapse formation. (Petzoldt & Sigrist, 2014)

Our Synaptogenesis Assay Services

Depending on a full range of in vitro cell culture models, scientists in Creative Biolabs develop synaptogenesis assay to evaluate the synapse formation & how the target compounds affect the synaptogenesis. Our synaptogenesis assay allows the recognition of the synapse, pre-synaptic vesicle, and post-synaptic puncta via the measurement of the localization of both the pre-synaptic marker and the post-synaptic marker. Statistic and image data are combined in a high-content analysis system to evaluate candidate compounds for drug screening.

Creative Biolabs provides global researchers with a customized target compound selecting program & related mechanism research with an in vitro synaptogenesis assay based on appropriate cell culture models. We guarantee our clients an efficient and reasonably priced assay for both academic and pharmaceutical use that facilitates the understanding of the mechanisms of synaptogenesis and promotes the drug development process. Our professional scientific team has a long-standing commitment to assisting researchers in synaptogenesis-related studies and answering questions about relevant expertise. For further information, please don't hesitate to contact us.

References

1. Petzoldt, A.G.; Sigrist, S.J. Synaptogenesis. Current Biology. 2014, 22(24): R1076-R1080.
2. Jakobsson, A.; et al. Three-dimensional functional human neuronal networks in uncompressed low-density electrospun fiber scaffolds. Nanomedicine: Nanotechnology, Biology and Medicine. 2017, 13(4): 1563-1573.