Microglia Activation Assay
Microglia, the resident immune cells of the central nervous system (CNS), play a pivotal role in maintaining brain homeostasis, immune surveillance, and responding to pathological insults. Dysregulation of microglial activation is implicated in various neurological disorders, including Alzheimer's disease, Parkinson's disease, and multiple sclerosis. Understanding the mechanisms underlying microglial activation is essential for developing targeted therapeutic interventions.
Creative Biolabs, with our extensive expertise and cutting-edge technologies, provides an array of microglia models and assays to facilitate groundbreaking research in this field.
In Vitro Microglia Models
Creative Biolabs offers a range of microglia models, each tailored to specific research needs:
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| Microglia Cell Lines | Primary Microglia | iPSC-Derived Microglia |
| Microglial cell lines provide immortalized cells derived from mice, rats, and humans, which have the advantages of easy maintenance, unrestricted proliferation ability, and abundant usability. | Methods for the isolation and single-cell culture of primary microglia from mice, rats, and humans are established. Using primary microglia allows researchers to precisely detect the role of specific genes in microglia activation in various disease models. | iPSC-derived microglia represent a cutting-edge model system, allowing researchers to generate patient-specific microglia for disease modeling and drug screening. We offer state-of-the-art iPSC-derived microglia services tailored to your research objectives. |
Microglia Assays
Creative Biolabs offers a comprehensive suite of professional and thorough microglia assay solutions tailored to meet your research needs:
- Markers Characterization: Microglia phenotype, as well as activation status, can be confirmed using a panel of validated markers: IBA1, CD11B, CD45, CD14, CX3CR1, GPR34, MERTK, C1Q, TREM2, SALL1, GLUT5, GAS6, CD68, TMEM119 and P2RY12.
- Morphology Characterization: One of the common indicators that define activated microglia is that microglia exhibit a broad morphological transformation of an "amoeboid" shape with enlarged cell bodies and shortened branches.
- Neuroinflammation Assay: Microglia are the main source of inflammatory factors in the CNS, and measuring microglial inflammation can further confirm their activation status. Our neuroinflammation assays encompass a wide range of markers, including NF-κB activation, TNF-α production, and IL-1β release. These assays provide a holistic view of the inflammatory response and guide the development of anti-inflammatory therapies.
- Phagocytosis Assay: Microglia are professional phagocytes. Regardless of physiological or pathological conditions, microglia constantly survey changes in the surrounding environment to respond quickly. Our advanced phagocytosis assays utilize cutting-edge imaging and flow cytometry techniques to evaluate microglia's phagocytic capacity. These assays are invaluable for studying clearance mechanisms in neurodegenerative diseases.
- Chemotaxis and Random Mobility Assay: In the CNS, microglia are constantly moving to check their niche, which is a kind of non-directional movement. At the same time, microglia also respond to chemokines for directional chemotaxis.
- Microglia Immunometabolism / Cytokine Profiling: Microglia require energy from metabolism to perform their immune functions. In addition to this, the activation of microglia also leads to changes in mitochondrial structure. Creative Biolabs offers high-throughput cytokine profiling assays, enabling precise measurement of pro-inflammatory and anti-inflammatory cytokines. These assays empower researchers to decipher the immune response dynamics within the central nervous system.
Creative Biolabs owns a professional neuroscience team that proposes the most professional and comprehensive technical solutions in microglial activation assay to help you improve scientific research and ultimately contribute to effective treatments for neurodegenerative diseases.
We provide efficient and comprehensive services with precise and reliable products to meet your research needs. Please contact us now to get quality service.
