Caenorhabditis elegans Model Deveopment Services

C. elegans (Caenorhabditis elegans) is a nematode worm that has been extensively used as a model organism in various fields of biological research, including neuroscience. Its small size, well-defined anatomy, short lifespan, and fully sequenced genome make it an ideal model for studying complex biological processes. Creative Biolabs offers a comprehensive range of services to facilitate research using C. elegans models.

CRISPR/Cas9 based C. elegans Gene Editing Service

Creative Biolabs employs cutting-edge genome editing techniques, such as CRISPR/Cas9, to facilitate precise modifications in the C. elegans genome. These techniques enable the introduction of targeted mutations, gene knockouts, gene replacements, and gene insertions. Our team of highly skilled scientists utilizes state-of-the-art technologies and protocols to ensure the successful creation of custom genome-edited C. elegans models.

Fig.1 Schematic representation of the CRISPR-Cas9 genome editing approach in C. elegans. (Kim, 2016)

Custom C. elegans Injection Service

Creative Biolabs recognizes the importance of precise delivery of genetic material into C. elegans for generating transgenic models. Our C. elegans Custom Injection Service offers a reliable and efficient method for introducing DNA constructs into C. elegans germlines or specific tissues of interest. Our custom injection mix service offers:

1. Collaborative design process: Work with our experts to design an injection mix that meets your needs.
2. Comprehensive reagent preparation: We provide all the necessary molecular components for your custom genetic edit.
3. Expert guidance and support: Receive assistance and advice throughout the process.
4. Timely delivery: We ensure prompt delivery of the injection mix to your location.

C. elegans MosSCI Service

Creative Biolabs also offers a MosSCI (Mos1-mediated Single-Copy Insertion) service for C. elegans. This service allows for the insertion of transgenes into a specific location in the genome, resulting in stable and predictable expression patterns. The MosSCI service offers the following benefits:

• Incorporate a humanized gene into the genome of C. elegans.
• Integrate your gene of interest into the genome while labeling it with a fluorescent tag.
• Integrate your gene of interest into the genome while tagging it with an immunotag.
• Investigate the expression of your gene of interest using a different promoter for control.

C. elegans Transgenic Models for Neurological Diseases

C. elegans has been widely used as a model organism for studying neurological diseases, due to its relatively simple nervous system and ease of genetic manipulation. Creative Biolabs offers a range of transgenic models for studying these diseases, such as:

• Transgenic C. elegans models for Parkinson's disease
• Transgenic C. elegans models for Alzheimer's disease
• Transgenic C. elegans models for Huntington's Disease
• Transgenic C. elegans models for Amyotrophic Lateral Sclerosis (ALS)
• Transgenic C. elegans models for Aging
• Transgenic C. elegans models for other Neurological Diseases including but not limited to spinocerebellar ataxias, frontotemporal dementia, and motor neuron diseases.

These models facilitate the exploration of disease mechanisms and the identification of potential therapeutic targets.

Fig.2 C. elegans models for Alzheimer's disease and Parkinson's disease. (Singh)

In addition to generating transgenic models, Creative Biolabs offers comprehensive phenotypic analysis services for C.elegans models. We provide detailed characterization of disease-related phenotypes, including locomotor abnormalities, lifespan analysis, protein aggregation assessment, and behavioral assays. Please feel free to contact us for more about our C.elegans models related services.

References

1. Kim, Hyun-Min & Colaiácovo, Monica. (2016). CRISPR-Cas9-Guided Genome Engineering in C. elegans: CRISPR-Cas9 Genome Engineering in C. elegans. 10.1002/cpmb.7.
2. Singh, Govind, and Payal Chauhan. "Caenorhabditis elegans as a Model System to Evaluate Neuroprotective Potential of Nano Formulations." Frontiers in Nanotechnology: 79.