Synaptic Imaging Assay Service

In the quest to understand the intricate workings of the brain, accurate synaptic analysis is paramount. Our synaptic imaging assay service provides a groundbreaking solution for researchers delving into cognitive mechanisms, learning behaviors, and neurodegenerative diseases. By combining state-of-the-art imaging technology with highly specific markers, we offer unparalleled precision and clarity in visualizing and quantifying synaptic function. For further information regarding the products and services provided, project-specific consultation, and pricing, please submit an inquiry here.

Our Synaptic Imaging Assays

Our services are based on in vitro neuronal models (mouse/rat hippocampal neurons, human iPSC-derived neurons) and use multiple fluorescent labeling and super-resolution imaging technology to accurately measure the following parameters

• Synapse Type Identification

Excitatory synapses: VGLUT1 (vesicular membrane protein) and PSD95 (postsynaptic marker) labeling.

Inhibitory synapses: Specific labeling with GAD65 (glutamate decarboxylase).

Supports Synaptobrevin2 (vesicle membrane anchor protein) detection to quantify the number, size and strength of synapses.

Fig.1 Fluorescence image of DA neurons (day 55) immunolabeled with VGLUT2 (green) and synapsin (magenta) antibodies.^{1, 3}

• Dynamic Functional Assessment

Synaptic strength: Combined with Synapsin IIa (vesicle aggregation protein) labeling to analyze the structural stability of synaptic active zones.

Ratio analysis: Calculate the balance of inhibitory/excitatory synapses to reveal changes in neural circuit plasticity.

Fig.2 vGluT1/GAT1 ratios.^{2, 3}

Why Choose Us

Multi-Dimensional Readouts	Cross-Model Validation Capabilities
Our service provides a multivariate analysis of synaptic activity, extending beyond standard mEPSC detection. It simultaneously resolves synaptic identity (excitatory/inhibitory), quantifies synaptic strength, and maps spatial distribution, enabling detailed investigation of complex neuronal mechanisms.	Our service supports cross-level research, facilitating validation across in vitro neuronal cultures and in vivo humanized mouse models, thereby streamlining preclinical translation.

Application

Our advanced synaptic imaging services are instrumental in driving research across critical neurological domains:

• Cognitive and Learning Sciences: We illuminate the synaptic mechanisms underlying learning and memory by precisely quantifying changes in synaptic density, type, and distribution.
• Neurodegenerative Disease Research: In conditions like Alzheimer's (AD) and Parkinson's (PD), our platform enables the detection of subtle synaptic loss and dysfunction, providing crucial insights for disease diagnosis, progression monitoring, and therapeutic development.
• Pharmaceutical Development: We accelerate drug discovery by providing robust assessments of candidate drug effects on synaptic function, facilitating rapid and accurate screening and efficacy evaluation.

Our team consists of experienced neuroscientists and technical experts who can provide comprehensive support, ranging from experimental design to data analysis. We invite you to visit our official website or contact our customer service team to learn more about our synaptic imaging assay service. We are eager to collaborate with you to advance the field of neuroscience research and drug development.

References

1. Fujise, Kenshiro, et al. "Synaptic vesicle characterization of iPSC-derived dopaminergic neurons provides insight into distinct secretory vesicle pools." npj Parkinson's Disease 11.1 (2025): 16.
2. Lauterborn, Julie C., et al. "Increased excitatory to inhibitory synaptic ratio in parietal cortex samples from individuals with Alzheimer's disease." Nature communications 12.1 (2021): 2603.
3. Distributed under Open Access license CC BY 4.0, without modification.