Aβ Induced Phagocytosis and Inflammation Assay

Neurological diseases cause huge social losses and heavy annual economic burdens, so it is urgent to develop new drugs. Equipped with world-leading technology platforms and professional scientific staff, Creative Biolabs provides novel Aβ induced phagocytosis and inflammation assay for our clients all over the world.

Amyloid beta and Alzheimer's Disease

Amyloid beta, also known as Aβ or Abeta, consists of 36-43 amino acids, it is the main component of the amyloid plaques in the brains of patients with Alzheimer's disease. Derived from the amyloid precursor protein (APP), Aβ is produced via cleavage by β-secretase and γ-secretase.

Alzheimer's disease is the most common type of dementia that induces the brain to shrink and brain cells to die. AD seriously reduces the quality of life of the elderly and brings a heavy economic burden. In this case, detection of future dementia risk with high sensitivity and specificity at the preclinical stage will largely prevent AD. The ultimate goal in detecting the risk of Alzheimer's disease (AD) is a blood test before neuronal damage occurs. However, existing amyloid-beta (Aβ) tests fail to detect the process leading to neurodegeneration. Recent studies have shown that multiple factors and signaling proteins in CSF or blood may be a rich source of diagnostic biomarkers. It should be noted that before formal use, efficacy needs to be tested in prospective studies. For example, a recently described flow cytometry-based detection of defective Aβ phagocytosis in AD patients has high sensitivity and specificity, but further experiments are necessary to apply it to the general population at risk of AD.

Fig.1 Differentially-expressed proteins in Aβ-stimulated or not stimulated PBMC's of an AD patient. (Fiala & Veerhuis, 2010)

Aβ induced Phagocytosis and Inflammation Assay

To help conduct more in-depth research on Aβ, now Creative Biolabs has established a platform for Aβ induced phagocytosis and inflammation assay using HCS imaging and qPCR detection. We are able to assess Aβ-induced phagocytosis and cytokines in mouse primary microglia and astrocytes. In completed projects, dose-dependent enhancement of Aβ phagocytosis and inhibition of IL-1β by positive compounds could be detected. Our advanced platform ensures the best achievement for every single step.

Equipped with world-leading technology platforms and professional scientific staff, Creative Biolabs is willing to provide the Aβ induced phagocytosis and inflammation assay for our clients. If you are interested in our services, please do not hesitate to contact us for more details.

Reference

1. Fiala, M. Veerhuis, R. Biomarkers of inflammation and amyloid-β phagocytosis in patients at risk of Alzheimer disease. Experimental gerontology. 2010, 45(1): 57-63.